羽扇豆醇对肝细胞肝癌的抑制作用研究  

Anti-tumor effects of lupeol on hepatocellular carcinoma

作　　者：梁勇[1] 胡命宝[1] 刘景[1] 

机构地区：[1]淮南联合大学医学系,安徽淮南232038

出　　处：《安徽医药》2013年第5期738-739,共2页Anhui Medical and Pharmaceutical Journal

基　　金：安徽省教育厅自然科学项目(No KJ2010B197);淮南联合大学自然科学项目(No LYB1109)

摘　　要：目的体外探讨羽扇豆醇对肝癌细胞系SMMC7721的抑制作用。方法利用MTT法检测羽扇豆醇对SMMC7721的毒性作用;流式细胞术检测羽扇豆醇作用SMMC7721细胞后对细胞周期及凋亡的影响。结果羽扇豆醇抑制SMMC7721的增殖,其能显著阻滞细胞在S期的聚集,促进细胞的凋亡。结论羽扇豆醇能抑制肝细胞肝癌(HCC)细胞的增殖,其作用机制可能与对诱导肝癌细胞凋亡和对细胞的S期阻滞相关。Objective To explore the inhibition effects of lupeol on hepatocellular carcinoma SMMC7721 in vitro. Methods In vitro in hibitions of luepol on proliferation of SMMC7721 ceils were analyzed by MTY assay;flow cytometry was used to detect lupeol induced ap optosis and cell cycle inhibition by characteristic morphological changes and biochemical features. Results The proliferation of SMMC7721 cells was dramatically inhibited by lupeol in a concentrationdependent manner, and 48h after exposed to lupeol, the IC50 val ue was 60 mol/L. Cell apoptosis and cycle block was induced by lupeol by flow cytometric analysis. Conclusion Lupeol shows signifi cant inhibitory effects on the growth of HCC cells in vitro.

关 键 词：羽扇豆醇 肝细胞肝癌 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]