黑米皮提取物对PC-3细胞增殖的抑制作用  

Inhibition effects of black rice pericarp extracts on cell proliferation of PC-3 cells

作　　者：姜伟伟 遇旭东 任国峰 

机构地区：[1]山东省龙口市疾病预防控制中心,龙口265701 [2]龙口市人民医院

出　　处：《卫生研究》2013年第3期474-477,482,共5页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30500407)

摘　　要：目的观察黑米皮提取物对人前列腺癌PC-3细胞增殖的抑制作用并探讨其机制。方法用不同浓度的黑米皮提取物(20,150和300μg/ml)处理PC-3细胞,通过MTT法、流式细胞仪、western blot法研究提取物对细胞的影响。结果处理组的细胞增殖率随着提取物作用浓度和时间的增加而降低(P<0.05);与对照组相比,处理组细胞凋亡率增加,差异有统计学意义(P<0.05);细胞周期中,G1期细胞百分比显著增多,S、G2-M期细胞显著减少,细胞被阻滞在G1-S期。与对照组相比,处理组细胞的p-p38、p-JNK的蛋白表达则随着提取物浓度的增加而增强。结论黑米皮提取物能够抑制人前列腺癌PC-3细胞的增殖,改变细胞周期的分布,激活JNK、p38通路,诱导细胞凋亡。Objective To observe the inhibitive effects of black rice pericarp extracts on cell proliferation of human prostate cancer cell PC-3 and to explore its effecting mechanism.Methods The black rice pericarp extract was used to treat the PC-3 cells.The inhibitory effect of black rice pericarp extract on cells proliferation of PC-3 was tested by MTT method.Cell apoptosis rates and cell cycle were measured by flow cytometric assay（FCM）.Western blot was used to study the protein expression levels of p38,p-p38,JNK,p-JNK.Results A dose-dependent and time-dependent proliferation inhibition of black rice pericarp extract was demonstrated in PC-3.The most prominent experiment condition was inhibitory concentration with 300μg/ml and treated for 72h.The experiment result of flow cytometry analysis demonstrates that the apoptosis rate of PC-3 cells increased along with the increasing of black rice pericarp extract concentration,and a G1-S cell cycle arrest was induced in a dose-dependent manner.After PC-3 cell was treated with black rice pericarp extract for 72h,the expressions of p-p38,p-JNK protein increased.Conclusion Black rice pericarp extract could inhibit proliferation,change the cell cycle distributions and induce apoptosis in human prostatic cancer cell PC-3.Its inhibitory effect may be through promoting activation of the JNK,p38 signaling pathway.These results suggest that black rice pericarp extract maybe has an inhibitory effect on prostatic cancer.

关 键 词：黑米皮提取物 前列腺癌细胞 凋亡 c-Jun氨基末端高激酶 P38 

分 类 号：R737.25[医药卫生—肿瘤] R979.1[医药卫生—临床医学]