舟山地区非小细胞肺癌患者Ⅲ型β微管蛋白mRNA表达及其与临床病理特征的关系  

Expression of TUBB3 mRNA in patients with non-small cell lung cancer and its relationship to clinical pathologic features in Zhoushan Island

作　　者：罗斌[1] 竺王玉[2] 张永奎[1] 

机构地区：[1]温州医学院附属舟山医院胸心外科,浙江省舟山316004 [2]温州医学院附属舟山医院中科院免疫基因组学实验室

出　　处：《中华航海医学与高气压医学杂志》2013年第2期115-118,136,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：浙江省科技厅项目（2011C37030）

摘　　要：目的研究舟山地区非小细胞肺癌（non—small cell lungcancer，NSCLC）患者肿瘤组织与正常肺组织中Ⅲ型β-微管蛋白（class Ⅲ β-tubulin，TUBB3）基因mRNA的表达差异及其与临床病理特征的关系。方法收集86例NSCLC患者手术切除肿瘤组织及相应正常肺组织，一步法逆转录实时荧光定量PCR（qRT—PCR）检测TUBB3mRNA表达，并分析其与临床病理特征的关系。结果TUBB3mRNA在NSCLC肿瘤组织中的表达（0．50±1．94）高于正常肺组织中的表达（0．18±0．57），差异有统计学意义（P=0．043），并且与临床病理类型有明显关系（P=0．035），腺癌是致TUBB3mRNA过表达的影响因素（P=0．042）；TUBB3mRNA表达情况与患者生存时间无明显相关性（P〉0．05）。结论舟山地区TUBB3mRNA在NSCLC肿瘤组织中呈高表达，其表达增高与肺腺癌有关，但与患者预后无关。Objective To investigate the differences in the expression of TUBB3 （class Ⅲ β-tubulin） mRNA between non-small cell lung cancer （NSCLC） tissues and normal lung tissues, and also to analyze its relationship with clinical pathologic features in Zhoushan Island. Methods Excised tumor tissue and normal lung tissue were collected from 86 cases of NSCLC. Then, one-step real-time qRT-PCR （quantitative PCR） was adopted to detect the expression level of TUBB3 mRNA. The relationship between TUBB3 mRNA in NSCLC and clinical pathologic features prognosis was analyzed. Results The expression level of TUBB3 mRNA in NSCLC tissue was higher than that in the normal lung tissue （P = 0. 043 ）. The overexpression of TUBB3 mRNA was obviously correlated with the pathological subtype （ P = 0. 035 ）, and the adenocarcinoma subtype was an important risk factor for the overexpression of TUBB3 mRNA （P = 0. 042）. In addition, TUBB3 mRNA expression was not directly correlated with the survival time of NSCLC patients （P 〉 0. 05 ）. Conclusions High expression of TUBB3 mRNA could be seen in NSCLC tissues in Zhoushan Island, which might be correlated with pulmonary adenocarcinoma, however, it might be irrelevant to the prognosis of patients.

关 键 词：Ⅲ型β-微管蛋白mRNA 非小细胞肺癌 舟山地区 

分 类 号：R734.2[医药卫生—肿瘤]