叶绿体分裂突变体cpd4中突变基因ARC5的鉴定与分析  

Identification and Analysis of the Mutant Gene ARC5 in a Chloroplast Division Mutant cpd4

作　　者：沈鑫曌[1] 刘含[1] 刘晓庆[1] 袁光孝[1] 高岳芳[1] 高宏波[1,2] 

机构地区：[1]北京林业大学,生物科学与技术学院,北京100083 [2]北京林业大学,林木育种国家工程实验室,北京100083

出　　处：《植物生理学报》2013年第5期477-484,共8页Plant Physiology Journal

基　　金：国家自然科学基金(30971439和J1103516);北京市自然科学基金(5102022)

摘　　要：cpd4是一个以Col为背景的拟南芥叶绿体分裂突变体,其突变表型与以Ler为背景的突变体arc5-1表型相似,即叶绿体数量明显减少,体积明显增大且形状常呈哑铃型。该突变未能对叶绿素含量造成明显影响。遗传分析显示其突变表型受隐性单基因控制。通过图位克隆的方法确定其突变性状是由ARC5基因突变引起的。该突变影响了ARC5基因mRNA的正常剪切,对mRNA的稳定性也造成严重影响。该工作为进一步研究ARC5在叶绿体分裂中的作用提供了有用的材料和信息。cpd4 （chlorol2last division 4） is a chloroplast division mutant isolated from Columbia background. It has a phenotype similar to that of arc5-1, a mutant from Landsberg erecta background, i.e., fewer and larger dumbbell shaped chloroplasts. The chlorophyll content was not obviously affected by the mutation in cpd4. Ge- netic analysis indicated that the mutant phenotype was controlled by a single recessive gene. The gene respon- sible for the mutant phenotype was identified to be ARC5 through a map-based cloning strategy. The mutation seriously affected the correct splicing and the stability of ARC5 mRNA. This work provides some useful mate- rials and information for further studying the function of ARC5.

关 键 词：叶绿体分裂突变体 cpa4 突变基因 ARC5 鉴定 分析 拟南芥 

分 类 号：Q943.2[生物学—植物学]