2型猪链球菌双组分调控系统SalK/SalR抗吞噬机制的初步研究  

Anti-phagocytosis mechanism of SalK /SalR,a two-component regulatory system of Streptococcus suis serotype 2

作　　者：付忠琳[1,2] 骈亚亚[2] 李雪琴[2] 郑玉玲[2] 马世良[1] 袁媛[2] 霍春月[3] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866 [2]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071 [3]首都医科大学燕京医学院,北京101300

出　　处：《细胞与分子免疫学杂志》2013年第6期570-573,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30870091)

摘　　要：目的确定双组分调控系统SalK/SalR在2型猪链球菌抵抗THP-1单核细胞来源的巨噬细胞(THP-MΦ)吞噬中的作用。方法透射电子显微镜观察野生株05ZYH33和突变株ΔsalKR荚膜的变化,革兰氏染色及免疫荧光方法观察猪链球菌与THP-MΦ细胞的相互作用,通过THP-MΦ细胞吞噬模型评价猪链球菌的抗吞噬能力。结果透射电子显微镜观察发现突变株ΔsalKR荚膜消失,革兰氏染色结果和免疫荧光标记实验显示salKR缺失导致更多的猪链球菌被THP-MΦ细胞吞噬,THP-MΦ细胞吞噬模型进一步证实突变株ΔsalKR比野生株更容易被THP-1细胞吞噬。结论双组分调控系统SalK/SalR通过调控荚膜的形成来抵抗THP-MΦ细胞的吞噬。Objective To determine the role of the two-component regulatory system （TCS） SalK/SalR in the resistance of Streptococcus suis serotype 2 （SS2） phagocytosed by THP-l-derived macrophages （THP-MФ））. Methods Using trans- mission electron microscopy （TEM）, the capsular differences between the wild-type strain 05ZYH33 and the mutant AsalKR were observed. The interactions of SS2 with THP-MФ） were monitored by Gram staining and immunofluorescence cytochem- istry. The anti-phagocytic activity of SS2 was evaluated by the construction of phagocytosis model of THP-MФ. Results The TEM showed that in the mutant AsalKR, the capsule was lost; the Gram staining and immunofluorescence imaging revealed that the absence of salKR caused more SS2 were engulfed by THP-MФ）. The phagocytosis model of THP-MФ cells further demonstrated that the mutant AsalKR was easier to be phagocytosed by THP-1 cells than the wild-type strain. Conclusion The SalK/SalR regulatory system resists the phagocytosis by THP-MФ through the capsular formation, but the mechanism of how it regulates the formation of the capsule needs further elucidation.

关 键 词：2型猪链球菌 双组分调控系统 荚膜 抗吞噬 

分 类 号：S852.61[农业科学—基础兽医学] Q256[农业科学—兽医学]