纳米银温敏原位凝胶的制备及流变学考察  

Preparation and Rheological Study of Silver Nanoparticles Thermosensitive Situ Gel

作　　者：郑英[1,2] 付再林[2] 陶蓉[3] 邹晓华[2] 陈素红[4] 吕圭源[1] 

机构地区：[1]浙江中医药大学,杭州310053 [2]解放军第117医院,杭州310004 [3]浙江医院,杭州310004 [4]温州医学院药学院,浙江温州325035

出　　处：《中国药房》2013年第21期1983-1985,共3页China Pharmacy

摘　　要：目的:制备纳米银(Ag-NPs)温敏原位凝胶并考察其流变学性质。方法:采用NaBH4还原AgNO3法制备Ag-NPs,通过四唑鎓盐(XTT)试验考察其对几种常见菌的抑菌效果;以不同质量比例的泊洛沙姆188(P188)-泊洛沙姆407(P407)为凝胶基质,制备Ag-NPs温敏原位凝胶,采用旋转流变仪、扫描电镜分别考察其流变学性质及该凝胶表征。结果:该Ag-NPs平均粒径为12.4nm,多分散系数为0.241,且10μg/mlAg-NPs对白色念珠菌的抑菌效果[抑制率(78.28±3.54)%]与1μg/ml两性霉素B[抑制率(83.33±4.55)%]相当(P=0.058),对金黄色葡萄球菌的抑菌效果[抑制率(88.66±2.94)%]与4u/ml庆大霉素[抑制率(89.92±3.78)%]相当(P=0.780)。Ag-NPs温敏原位凝胶室温下为液体,升高至相变温度(Tg)则迅速呈半固态,并随P407质量比例的增加Tg下降,且pH对其无显著影响;当P407与P188比例分别为8%:22%、10%:20%、12%:18%、15%:15%时,其对应Tg分别为34.8、31.2、28.7、25.6℃。结论:所制备的Ag-NPs温敏原位凝胶具有良好的流变学特性及较强的抑菌活性。OBJECTIVE：To prepare Sliver nanoparticles（Ag-NPs）thermosensitive situ gel and investigate its rheological property.METHODS：Ag-NPs were prepared by the borohydride reduction of AgNO3.XTT test was used to investigate the antibacterial effects of Ag-NPs on several common bacterials.The gels were prepared by dissolving the mixture of different proportions of P188 and P407 in the Ag-NPs solution.The rheological property and microstructural analysis were tested via rheometer and scanning electron microscope.RESULTS：Mean particle size of Ag-NPs was 12.4 nm,and polydispersity index was 0.241.The anti-bacterial effects of 10 μg/ml Ag-NPs to Monillia albicans [inhibitory rate of（78.28±3.54）%] was equal to that of 1 μg/ml Amphotericin B [inhibitory rate of（83.33±4.55）%]（P=0.058）;the anti-bacterial effects to Staphylococcus aureus [inhibitory rate of（88.66±2.94）%] was equal to that of 4 u/ml Gentamicin [inhibitory rate of（89.92±3.78）%]（P=0.780）.Ag-NPs thermosensitive situ gel was liquid at room temperature,and represented as semi-solid when increasing to phase-transition temperature（Tg）.As the increase of P407 proportion,Tg decreased.pH had no significant effect on it.P407：P188=8%：22%,10%：20%,12%：18%,15%：15% corresponded to Tg of 34.8,31.2,28.7,25.6 ℃.CONCLUSIONS：Prepared Ag-NPs thermosensitive situ gel shows sound rheological properties and anti-bacterial activity.

关 键 词：纳米银 制备 泊洛沙姆 温敏原位凝胶 流变学 抑菌活性 

分 类 号：R944[医药卫生—药剂学]