鼻咽癌移植瘤Annexin A3和Nm23-H1蛋白表达与放射敏感相关性的研究  

Annexin A3,Nm23-H1 protein expression in different radiosensitivity xenograft model of nasopharyngeal carcinoma

作　　者：曲颂[1] 李小宇[1] 朱小东[1] 葛莲英[1] 李龄[1] 梁世雄[1] 苏芳[1] 郭亚[1] 李佳荃[2] 

机构地区：[1]广西医科大学附属肿瘤医院放疗科,广西南宁530021 [2]广西医科大学医学科学实验中心,广西南宁530021

出　　处：《中华肿瘤防治杂志》2013年第11期801-805,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30860329);广西自然科学基金(桂科自0832229);广西高等学校重大科研资助项目(201101D004);广西医科大学博士科研项目(304186);广西研究生教育创新计划项目(2010105981002M218)

摘　　要：目的:探讨不同放射敏感性鼻咽癌移植瘤中Annexin A3和Nm23-H1蛋白的表达及意义。方法:将不同放射敏感性鼻咽癌细胞CNE-2R和CNE-2分别注射到裸鼠后肢皮下成瘤。成瘤后以X射线分次照射16Gy,计算肿瘤体积及肿瘤倍增时间。采用免疫组化法检测移植瘤中Annexin A3和Nm23-H1蛋白的表达水平。结果:未受照射CNE-2R和CNE-2移植瘤倍增时间分别为4.8和3.9d,受照射CNE-2R和CNE-2移植瘤倍增时间分别为6.2和17.1d。未受照射CNE-2R移植瘤组织Annexin A3蛋白表达明显低于未受照射CNE-2移植瘤组织,MOD值分别为0.035±0.012和0.062±0.009,P=0.000;受照射CNE-2R移植瘤组织明显低于受照射CNE-2移植瘤组织,MOD值分别为0.029±0.007和0.051±0.009,P=0.000。未受照射CNE-2R移植瘤组织Nm23-H1蛋白明显高于未受照射CNE-2移植瘤组织,MOD值分别为0.056±0.007和0.043±0.007,P=0.022;受照射CNE-2R移植瘤组织明显高于受照射CNE-2移植瘤组织,MOD值分别为0.079±0.009和0.046±0.007,P=0.000。结论:CNE-2R移植瘤组织Annexin A3蛋白明显低于CNE-2移植瘤组织,受照射CNE-2R移植瘤组织Nm23-H1蛋白明显高于受照射CNE-2移植瘤组织,与体外实验结论一致,提示与鼻咽癌放射抗拒有关,可作为鼻咽癌放射治疗的新标志。OBJECTIVE：To investigate protein expressions of Annexin A3 and Nm23-H1 in different radiosensitivity of human nasopharyngeal carcinoma （NPC） xenografts. METHODS： CNE-2R and CNE-2 cells were subcutaneously injec- ted into hind legs of nude mice. Tumors-bearing mice received X-ray irradiation, 16 Gy in 4 fractions delivered over 8 days. Tumor volumes and tumor doubling times were calculated. Tumor tissues were examined by immunohistochemical staining for Annexin A3 and Nm23-H1 protein expressions. RESULTS： Tumor doubling times of the NIR-CNE-2R and NIR-CNE-2 tumors were 4. 8 days and 3. 9 days, respectively. Tumor doubling times of the IR-CNE-2R, IR-CNE-2 tumors were 6.2 days and 17. 1 days, respectively. Annexin A3 protein expression was significantly lower in the NIR- CNE-2R tumors than that in the NIR-CNE-2 tumors, and MOD value were 0. 035 ±0. 012 and 0. 062 ±0. 009 （P = 0. 000）. It was down-regulated in the IR-CNE-2R tumors compared to the IR-CNE-2 tumors, and MOD value were 0. 029±0. 007 and 0. 051±0. 009 （P=0. 000）. Nm23-H1 protein expression was higher in the NIR-CNE-2R tumors than that in the NIR-CNE-2 tumors,and MOD value were 0. 056±0. 007 and 0. 043±0. 007 （P=0. 022）. It was significantly up-regulated in the IR-CNE-2R tumors than that of the IR-CNE-2 tumors, and MOD value were 0. 079 ± 0. 009 and 0. 046± 0. 007 （P= 0. 000）. CONCLUSIONS： Annexin A3 protein expression is significantly lower in the CNE-2R tumorsthan that in the CNE-2 tumors, and Nm23-H1 expression is significantly higher, which is in conformance with our previous in vitro study. Annexin A3 and Nm23-H1 protein expressions are related to radioresistance of NPC,and they can be new biomakers in radiotherapy of NPC.

关 键 词：鼻咽肿瘤 移植瘤 放射敏感性 小鼠 裸 

分 类 号：R739.63[医药卫生—肿瘤]