CLA对奶牛乳腺上皮细胞脂肪酸合成相关酶的影响  

Effects of conjugated linoleic acids on key enzymes in fatty acid synthesis in bovine mammary epithelial cells

作　　者：王晓宇[1,2] 刘晶[1,2] 侯俊财[1,2] 姜成刚[3] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学食品学院,哈尔滨150030 [3]中国农业科学院哈尔滨兽医研究所,哈尔滨150001

出　　处：《黑龙江畜牧兽医》2013年第6期1-5,188,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30901029)

摘　　要：为了研究体外添加c9,t11共轭亚油酸(CLA)和t10,c12 CLA对奶牛乳腺细胞(BMECs)中脂肪酸(FA)合成关键酶基因mRNA转录和SCD酶指数的影响,试验采用组织块分离培养BMECs,两种CLA浓度分别为0,50,100,150μmol/L,作用细胞24 h后采用荧光定量PCR(QRT-PCR)对目的基因进行相对定量。结果表明:与对照组相比,50μmol/L c9,t11 CLA显著抑制BMECs中的ACC、FAS、D5和D6基因mRNA,但150μmol/L却显著促进;50μmol/L t10,c12 CLA显著上调了ACC、SCD5、D5和D6基因的mRNA转录水平,对FAS转录无影响(P>0.05),但150μmol/L显著受到抑制;CLA均抑制SCD1的转录,促进SCD5转录。C9,t11 CLA使SCD酶指数显著增加,t10,c12 CLA则相反。To investigate the effects of in vitro addition of c9, tll and tl0, c12 conjugated linoleic acids （CLA） on mRNA transcription of genes for key enzymes in fatty acid synthesis and SCD indexes in bovine mammary epithelial cells （BMECs）. The BMECs was isolated and cultured in vitro with the explants culture method. After the cells were incubated with CLA for 24 h, in which the concentrations of both CLA were 0, 50, 100 and 150 μmol/L, respectively for 24 h, the target genes were relatively quantified by fluorescence quantitative PCR. The result showed that compared with the control, 50 μmol/L c9, t11 CLA significantly inhibited the mRNA levels of ACC, FAS, D5 and D6 genes, but 150 μmol/L significantly promoted the mRNA levels;50 μmol/L t10, c12 CLA significantly upregnlated the mRNA transcription levels of ACC, SCD5, D5 and D6 genes, which had no effect on FAS（ P 〉 0.05 ） ; but FAS was significantly inhibited at 150 μmol/L. Meanwhile, the two CLA significantly inhibited the transcription level of SCD1, but promoted the level of SCDS. C9, t11 CLA significantly increased the SCD indexes （P 〈0.05） ,but tl0, c12 CLA significantly reduced the SCD indexes.

关 键 词：共轭亚油酸(CLA) 奶牛乳腺上皮细胞(BMEC) 荧光定量PCR 转录 SCD酶指数 

分 类 号：S823.91[农业科学—畜牧学]