正交法检测环境因素对空肠弯曲杆菌生物膜形成的影响  

Detection of the influences of environmental factors on the biofilm formation of Campylobacter jejuni with orthogonal experiment

作　　者：秦晓庆[1] 张蕾[1] 赵树臣[1] 侯振中[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《黑龙江畜牧兽医》2013年第6期29-31,191,共4页Heilongjiang Animal Science And veterinary Medicine

摘　　要：为了研究各种环境因素对空肠弯曲杆菌生物膜形成的影响,试验采用正交试验的方法,结合光学显微镜和扫描电镜,综合分析空气条件、培养基类型、温度这三种环境因素对空肠弯曲杆菌生物膜形成水平的影响。结果表明:三种环境因素中对空肠弯曲杆菌形成影响的顺序是空气条件>温度>培养基类型,每种环境因素在不同水平时,生物膜形成的量也有差异。说明适当的有氧环境及营养的相对缺乏更有利于空肠弯曲杆菌生物膜的形成。To study the influences of various environmental factors on the biofilm formation of Campylobacter jejuni, the orthogonal tests were used to synthetically analyze the influences of the air condition, type of medium and temperature on the biofilm formation with the optical microscopy and scanning electron microscopy. The results showed that the influences of three factors in descending order were the air condition, temperature, and type of medium. The biofilm - forming capacities were also different with each of these factors at different levels. The results indicate that the appropriate aerobic and oligotrophic environments were more conducive to the biofilm formation.

关 键 词：空肠弯曲杆菌 生物膜 影响因素 正交试验 结晶紫 扫描电镜 

分 类 号：S852.613[农业科学—基础兽医学]