孕鼠补充牛磺酸对宫内生长受限胎鼠脑组织Ras同源基因-Rho相关卷曲螺旋蛋白激酶通路关键信号分子表达的影响  

Influence of antenatal taurine on expression of key signaling molecule of Ras homolog gene-Rho associated coiled-coil forming protein kinase-proliferating cell nuclear antigen pathway in fetal rat brain with intrauterine growth restriction

作　　者：王晓凤[1] 刘颖[1] 刘敬[1] 韩涛[1] 任晓暾[1] 马秀伟[1] 

机构地区：[1]北京军区总医院附属八一儿童医院新生儿重症监护中心,100700

出　　处：《中华实用儿科临床杂志》2013年第8期617-620,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金（81170577）

摘　　要：目的观察孕鼠补充牛磺酸对宫内生长受限（IUGR）胎鼠脑组织Ras同源基因-Rho相关卷曲螺旋蛋白激酶（Rho—ROCK）通路中关键信号分子及增殖细胞核抗原（PCNA）表达的影响，以探讨牛磺酸是否可通过该信号途径促进IUGR胎鼠皮质神经元增殖。方法采取低蛋白饮食法建立IUGR模型，将30只孕鼠按妊娠顺序分为对照组、IUGR模型组（IUGR组）及IUGR＋孕鼠补充牛磺酸组（IUGR＋牛磺酸组），牛磺酸组自妊娠第12天开始于饲料中添加牛磺酸300mg／（kg·d）直至自然分娩。采用实时定量（Real．time）-PCR法检测各组胎鼠脑组织中Ras同源基因（Rho基因）、Rho相关卷曲螺旋蛋白激酶基因（ROCK2基因）及PCNA基因mR．NA水平，同时采用免疫组织化学法检测各组胎鼠脑组织PCNA蛋白的表达。结果1．IUGR组、牛磺酸组与对照组胎鼠脑组织鼢oA、ROCK2及PCNAmRNA水平：肋oAmRNA分别为1．757±0．041、1．498±0．011和1．000±0．000（P〈0．05），ROCK2mRNA分男1为1．548±0．231、1．094±0．049和1．000±0．000（P〈0．05）．PCNAmRNA分别为2．007±0．800、3．034±0．670和1．000±0．000（P〈0．05）。2．IUGR组、牛磺酸组与对照组胎鼠脑组织PCNA免疫组织化学阳性细胞数分别为（22．24±6．17）、（77．80±14．60）和（11．30±3．18）个／高倍视野（P〈0．05）。结论孕鼠补充牛磺酸可能通过抑制Rho—ROCK信号途径而发挥对IUGR胎鼠的脑保护作用及促进IUGR胎鼠脑发育，为产前补充牛磺酸促进IUGR脑发育提供了理论依据。Objective To explore the influence of antenatal taurine supplementation on the expression of key signaling molecule of Ras homolog gene-Rho associated coiled-coil forming protein kinase-proliferating cell nuclear anti- gen（Rho-ROCK-PCNAR） pathway in fetal rat brain with intrauterine growth restriction（ IUGR）, and to understand whether or not taurine can improve neuron regeneration in IUGR fetal rats by this signaling pathway. Methods Thirty pregnant rats were randomly divided into 3 groups ： control group, IUGR model（ IUGR group） and IUGR ＋ antenatal tau- fine supplements group（ IUGR ＋ taurine group）. Taurine was added to the diet of IUGR ＋ taurine group at a dose of 300 mg/（ kg . d） from 12 days after conception until natural delivery. The level of mRNA expressions of Ras homolog gene A（ RhoA）, Rho-associated coiled coil-forming protein kinase 2 gene（ ROCK2 gene） and PCNA gene were detected by Real time-PCR. The PCNA positive cell counts were detected by immunohistochemistry. Results 1. The level of RhoA, ROCK2 and PCNA mRNA in the IUGR group, IUGR ＋ taurine group and control group were respectively ： RhoA mRNA 1.757±0.041,1.498 ±0.011 and 1.000 ±0. 000（P〈0. 05）;ROCK2 mRNA 1.548±0.231,1.094±0.049 and 1. 000±0. 000（P 〈0.05） ;PCNA mRNA 2. 007 ± 0. 800,3. 034± 0.670 and 1. 000± 0.000（P 〈 0.05 ）. 2. The PCNA positive cell counts in control group, IUGR group and IUGR ＋ taurine group were respectively 11.30±3.18,22.24 ±6. 17 and 77.80 ± 14.60 （ P 〈 0.05 ）. Conclusions Antenatal supplementation of taurine can inhibit the expression of key sig- naling molecule of Rho-ROCK pathway and improve the expression of PCNA in IUGR fetal brain ,which provides a further theoretical basis for the application of antenatal taurine to improve IUGR fetal brain development.

关 键 词：宫内生长受限 牛磺酸 Ras同源基因-Rho相关卷曲螺旋蛋白激酶信号通路 胚胎 鼠 

分 类 号：R7[医药卫生—临床医学]