Twist1对人乳腺癌细胞E-cadher in表达及亚细胞定位的影响  

Effect of Twist1 on E-cadherin expression and subcellular location of human breast carcinoma cell line

作　　者：张冲[1] 孙保存[1,2] 张丹芳[1] 刘芳[1] 董学易[1] 刘志勇[2] 车娜[1] 赵秀兰[1] 

机构地区：[1]天津医科大学病理教研室,天津市300070 [2]天津医科大学附属肿瘤医院病理科

出　　处：《中国肿瘤临床》2013年第9期509-512,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金重点项目(编号:81230050);国家自然科学基金青年科学基金项目(编号:81101724);国家自然科学基金面上项目(编号:81172046)资助~~

摘　　要：目的:探讨转录调控因子Twist1对人乳腺癌MCF-7细胞E-cadherin表达的影响。方法:上调人乳腺癌MCF-7细胞中Twist1的表达,利用RT-PCR,Western blot,细胞免疫荧光检测分析上调Twist1对E-cadherin表达的影响;划痕实验分析对细胞侵袭运动能力的影响;三维培养观察Twist1对MCF-7细胞成血管能力的影响。结果:MCF-7细胞过表达Twist1抑制细胞E-cadherin的表达,并且导致其亚细胞定位异常,细胞迁移运动能力增强,细胞成血管能力增强。结论:Twist1能抑制MCF-7细胞E-cadherin的表达,并增强其侵袭迁移及血管生成能力。Objective：This study aims to investigate the effect of Twist1 on human breast carcinoma E-cadherin. Methods： pcD-NA-Twist1 was transfected into human breast carcinoma cell line MCF-7. The expression of E-cadherin was detected via real-time polymerase chain reaction and immunofluorescence staining. Wound-healing assay was performed to examine the migration ability of cells, and the 3D culture was used to observe vasculogenic mimicry （VM） formation. Results：MCF-7 cells overexpressing Twist1 resulted in the suppression of E-cadherin expression and abnormal subcellular location. Migration ability and VM formation were also enhanced. Conclusion：Twist1 prevented E-cadherin expression as well as promoted migration ability and VM formation in human breast carcinoma cell line.

关 键 词：TWIST1 E-CADHERIN 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]