原发性肝细胞癌切除术中快速处理肿瘤细胞污染器械的方法研究  

A study on the rapid eradication of tumor cells on the surgical tools used in hepatocellular carcinoma re-section

作　　者：喻晓芬[1] 叶再元[1] 胡弦琴[1] 马英玉[2] 张琴芳[1] 

机构地区：[1]浙江省人民医院手术室,杭州310014 [2]浙江省人民医院实验室,杭州310014

出　　处：《中华肝胆外科杂志》2013年第5期329-331,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：浙江省卫生厅基金资助项目（2013KYA011）

摘　　要：目的探讨原发性肝细胞癌切除术中快速灭活污染器械肿瘤细胞的方法。方法用37℃生理盐水浸泡并刷洗原发性肝细胞癌切除术中可疑污染器械。浸泡液经低速离心后，提取并培养沉淀的肿瘤细胞。使用不同温度的蒸馏水或生理盐水浸泡肿瘤细胞不同时间后，HE染色倒置显微镜下观察细胞的形态。结果在55℃蒸馏水中浸泡60S后，肿瘤细胞肿胀，胞膜消失，细胞核体积缩小，核内染色质浓缩，染色变深，部分细胞裂解脱落。肿瘤细胞经55℃蒸馏水浸泡60s后更换含胎牛血清（FBS）的RPMI1640培养液再培养24h，未发现贴壁生长的有活性细胞。而在55℃的生理盐水中浸泡60S后，肿瘤细胞核膜形态未见明显改变。结论原发性肝细胞癌切除术中可疑污染器械在55℃蒸馏水中浸泡60S，肿瘤细胞能被全部灭活。Objective This study investigated a method to rapidly inactivate tumor cells on sur gical instruments intraoperatively. Methods Tumor cells were collected by immersing and washing the surgical instruments in 37 ℃ saline. The precipitation was collected by low speed centrifugation and then was cultured to harvest the tumor cells. The tumor cells were immersed in saline and distilled water of different temperatures for different duration of time. Inverted microscopy was used to investi- gate the changes in morphology. Results After immersion in 55℃ distilled water for 60 seconds, the tumor cells were swollen, the cell membranes disappeared, the sizes of the nuclei were reduced, the chromatin was condensed, and some cells lysed and separated from each other. Additionally, these tumor cells floated in the culture medium and lacked any living cells adhering to the walls of the bottle. In the group of tumor cells treated with 55 ℃ saline for 60 seconds, there were no obvious morpholog ical changes of the tumor cell or nucleus. Conclusion The intraoperative immersion of surgical instru- ments in 55 ℃ distilled water for 60 seconds could completely inactivate tumor cells.

关 键 词：原发性肝细胞癌 无瘤技术 手术器械 生理盐水 蒸馏水 

分 类 号：R735.7[医药卫生—肿瘤]