急性白血病血清蛋白质谱特征研究  

Study on serum protein mass spectrometric characteristics of acute leukemia

作　　者：郑瑞玑[1] 马旭东[1] 

机构地区：[1]福建医科大学附属漳州市医院血液内科,363000

出　　处：《中华血液学杂志》2013年第5期426-429,共4页Chinese Journal of Hematology

摘　　要：目的应用差异蛋白质谱的方法研究急性白血病患者与健康人血清中蛋白质谱的表达差异。方法提取51例急性白血病患者和10名健康志愿者的外周血血清，去除血清样品中的高丰度蛋白，用双向凝胶电泳方法进行血清低丰度蛋白质的分离，找出急性白血病患者与健康人血清中的差异蛋白点，切出差异蛋白点后进行脱色、胶内酶切、肽段提取，表面增强激光解吸一电离飞行时间质谱（MALDITOF／TOFMS）进行分析获得肽质量指纹图。使用蛋白质数据库MSDB提供的Masort检索程序进行评价。结果成组t检验结果显示，与健康对照组比较，急性白血病患者血清有19个差异有统计学意义的异常表达蛋白点（P〈0．05），经过质谱鉴定0【1一胰蛋白酶抑制剂（P〈0．01）、前白蛋白（P〈0．01）、胰蛋白酶抑制剂（P〈0．01）、载脂蛋白E（P〈0．01）、载脂蛋白A-Ⅳ（P〈0．01）为表达下降，而视黄醇结合蛋白（P〈0．05）、结合珠蛋白HP2（P〈0．05）、血清外源性凝集素（P〈0．05）、H因子同源物（P〈0．05）、血清淀粉样蛋白A1（P〈0．01）为表达上调，进一步分层分析发现血清外源性凝集素表达高的急性白血病患者治疗效果较差。结论急性白血病患者外周血血清中存在多种蛋白质的差异表达，血清外源性凝集素的表达差异与治疗效果相关，这些蛋白的差异表达可作为新的急性白血病诊断标志或者预后指标。Objective To study the serum protein differential expression in acute leukemia patients and healthy control by differential protein mass spectrometry. Methods Serum proteins of 51 acute leukemia （AL） patients and 10 healthy donors were extracted from their peripheral blood. After removing high abundance protein, serum low abundance proteins were separated by two dimensional gel electrophoresis, the differences of serum proteins in AL patients and healthy human were identified. The protein spots with differential expression were cut out and then undergone bleaching, gel digestion and peptide extraction. The peptide mass fingerprint analysis was performed by using MALDI TOF/TOF MS. The protein database MSDB Masort retrieval program was used to evaluate the resuhs. Results Using Student＇ s t test, 19 statistically significant abnormal expression proteins in the serum of AL patients were found compared with the healthy controls （ P 〈 0.05 ）. The expression of al-trypsin inhibitor （ P 〈 0.01 ） , prealbumin （ P 〈 0.01 ） , trypsin inhibitor （ P 〈 0.01 ） , apolipoprotein E （ P 〈 0.01 ） and apolipoprotein A-IV （ P 〈 0.01 ） decreased, while retinol binding protein （ P 〈 0.05 ）, globin HP2 （ P 〈 0.05 ）, serum lectin （ P 〈 0.05 ）, H factor homologue protein （P 〈 0.05 ） and serum amyloid A1 （P 〈 0.01 ） increased. Further stratified analysis found that high serum lectin expression in AL patient resulted in poor outcomes. Conclusion There are a variety of serum proteins with differential expression in peripheral blood of AL patients. The differential expression of serum lectin is related to the therapeutic effect. The differential expression of these proteins can be used as a new diagnosis marker or prognostic indicator for acute leukemia.

关 键 词：白血病 急性 蛋白质谱 血清 

分 类 号：R733[医药卫生—肿瘤]