CAST基因多态性与西门塔尔杂交牛胴体和肉质性状的相关性分析  

Relationship between CAST gene polymorphisms and carcass and meat quality traits of Chinese Simmental-cross steers

作　　者：沈冰蕾[1,2] 刘博洋[2] 赵志辉[2] 杨润军[2] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国生物制品学杂志》2013年第5期653-656,共4页Chinese Journal of Biologicals

基　　金：国家科技支撑计划项目(2007BAD55B01);国家"863"项目(2008AA101010)

摘　　要：目的分析钙蛋白酶抑制蛋白(Calpastatin,CAST)基因多态性与西门塔尔杂交牛胴体和肉质性状的相关性。方法选取95头西门塔尔杂交牛,采用限制性片段长度多态性聚合酶链反应(Restriction fragment length polymor-phism-polymerase chain reaction,RFLP-PCR)对CAST基因外显子1(Exon1)和外显子5(Exon5)区域的片段的多态性进行检测,并对突变位点与西门塔尔肉牛胴体和肉质性状的相关性进行分析。结果在所分析的片段中,仅引物4扩增的片段(Exon1)存在一个G→C碱基的置换突变(E1 256 g﹥c),为同义突变;该突变位点为RasⅠ酶的自然酶切位点,酶切后存在GG、GC和CC 3种基因型,CC基因型与西门塔尔牛的净肉率性状显著相关(P﹤0.05),而对其他胴体和肉质性状无显著影响。结论 CAST基因Exon1处存在的突变位点与西门塔尔肉牛的净肉率显著相关,因此该位点将有助于筛选与牛肉质相关的辅助选择标记,该基因将为鉴定与肉质相关的功能基因提供参考。Objective To analyze the relationship between calpastatin（CAST）gene polymorphism and carcass and meat quality traits of Chinese Simmental-cross steers.Methods The polymorphisms of Exon1 and Exon5 of CAST gene of 95 Chinese Simmental-cross steers were analyzed by restriction fragment length polymorphism polymerase chain reaction（PCR-RFLP）,based on which the relationship between mutation site and carcass and meat quality traits was analyzed. Results In the gene fragments analyzed,only one base substitution of G→C（E1 256 g c）was observed in Exon1 amplified by using primer 4,which was a samesense mutation.The site was the restriction site of Ras Ⅰ.Three genotypes were obtained after digestion with RasⅠ.Genotype CC was significantly related to the net meat weight（NMW）（P 0.05）,while showed no significant effect on other carcass and meat quality traits.Conclusion The mutation site at Exon1 of CAST gene was significantly related to NMW of Chinese Simmental-cross steers,which was helpful to screening of meat quality-associated assistant marker.CAST gene will provide a reference for identification of functional gene associated with meat quality.

关 键 词：钙蛋白酶抑制蛋白 西门塔尔牛 胴体性状 肉质性状 多态性 

分 类 号：S823.92[农业科学—畜牧学]