粗叶悬钩子叶中多酚类与三萜类化合物的研究  

Polyphenols and Triterpenoids in Rubus aleaefolius Poir. Leaves

作　　者：郭依俐[1] 唐铁鑫[1] 杨得坡[1,2] 岑山 朱龙平[1,2] 徐新军[1,2] 

机构地区：[1]中山大学药学院天然药物与中药研究所,广州510006 [2]广东省现代中药工程技术研究开发中心,广州510006

出　　处：《植物科学学报》2013年第2期191-197,共7页Plant Science Journal

基　　金：中华人民共和国科学技术部国际科技合作项目(2009DFA31230):基于澳大利亚TGA的中药(南药)质量标准与注册研究;广东省教育部产学研结合项目(2010B090400533):中药化学对照品制备关键技术及商业化运营平台的构建

摘　　要：采用高效液相色谱-串联质谱(HPLC-MS/MS)联用技术,对中国南方民间用于治疗肝损伤和癌症的药材粗叶悬钩子(Rubus aleaefolius Poir.)叶中的多酚类和三萜类成分进行了分析。通过与文献数据和部分对照品的紫外光谱、负离子模式下的一级和二级质谱信息进行比较,从粗叶悬钩子叶中共分析了18个化合物,鉴定了其中2个多酚类化合物,推测了9个多酚类和7个三萜类化合物的可能结构,并对三萜类化合物的质谱裂解途径进行了解析。基于对三萜类化合物裂解规律的研究,推测了1个三萜,即3-对香豆酰氧基-19α-羟基熊果酸。芦丁、绿原酸和3-对香豆酰氧基-19α-羟基熊果酸在本植物中属首次报道。Rubus aleaefofius Poir. （Rosaceae）, ＂Cu Ye Xuan Gou Zi＂, is used as Chinese medicine to treat liver injury and cancer in the south of China. Polyphenols and triterpenoids in its leaves were analyzed by high performance liquid chromatography coupled with diode-array detector and tandem mass spectrometry. Compound structures were tentatively identified by comparing ultraviolet （UV） spectra and mass spectra with those of certain authentic samples and literature. Eighteen compounds were analyzed in the leaves of R. aleaefofius, among which two polyphenols were definitely identified, and nine polyphenols and seven triterpenoids were tentatively identified. Additionally, fragmentation patterns of main fragment ions for triterpenoids in negative ion mode were proposed and summarized. According to these patterns, a triterpe- noid, 3-O-p-coumaroyl-19α-hydroxyursolic acid was tentatively identified. Rutin, chlorogenic acid and 3-O-p-coumaroyl-19α-hydroxyursolic acid were reported in the R. aleaefolius leaves for the first time.

关 键 词：粗叶悬钩子叶 高效液相色谱-串联质谱 多酚 三萜 

分 类 号：Q946[生物学—植物学]