提莫菲维小麦与光稃野燕麦远缘杂交后代花粉母细胞减数分裂行为分析  

Chromosome Behavior of Pollen Mother Cell Meiosis in the Hybrid Progenies from Triticum timopheevi×Avena fatua L. var. glabrata Pat

作　　者：安洪周[1] 胡梅[1] 郑会敏[1] 张素勤[1] 张庆勤[1] 

机构地区：[1]贵州大学农学院,贵阳550025

出　　处：《西北植物学报》2013年第2期295-300,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31160224);科技部农业科技成果转化资金(2010GB2F200429);农业部计划项目(2011-A2521-520111-A0105-013);贵州省农业攻关项目(黔科合NY字[2008]3017;黔科合带帽字[2009]5002)

摘　　要：通过普通细胞学和基因组原位杂交(GISH)研究提莫菲维小麦(Triticum timopheevi)与光稃野燕麦(Avenafatua L.var.glabrata Pat)远缘杂交后代花粉母细胞染色体减数分裂行为。结果表明,该F3株系花粉母细胞的减数分裂指数为87.46,表现出一定的遗传不稳定性。GISH分析发现该F3株系中期I细胞中有4对红色杂交信号,且游离的单价体上各有1个杂交信号;后期Ⅰ、后期Ⅱ和末期Ⅱ出现落后染色体、染色体桥、微核等现象,分别占观察数的10.58%、1.92%和12.36%,这些异常现象可能是由于光稃野燕麦遗传物质干扰了小麦同源染色体或部分同源染色体的正常配对造成的。The chromosomic meiotic behavior of pollen mother cells（PMCs） of the hybrid progenies from Triticum timopheevi×Avena fatua L.var.glabrata Pat was researched by general cytology and genomic in situ hybridization（GISH） in the present study.The results showed that the PMCs meiotic index of the F3 line was 87.46,indicating some genetic instability.4 pairs of red hybridization signals were found in the PMCs at the metaphase I by GISH analysis,and there was a red signal in each dissociative univalent.Some lagging chromosome,chromosome bridges and micronuclei were discovered at the anaphase Ⅰ,anaphase Ⅱ and telophase Ⅱ,with the percentage of 10.58%,1.92% and 12.36%,respectively.Wheat homologues or homoeologues normally pairing was interfered by the wild oat chromatin might lead to these abnormal phenomena.

关 键 词：小麦 野燕麦 减数分裂 染色体行为 基因组原位杂交 

分 类 号：Q321[生物学—遗传学]