基底细胞样乳腺癌细胞HCC1937中p21^wafl抗凋亡机制分析  

Anti-apoptotic mechanism for p21^wafl in human basal like breast cancer cell line HCC1937

作　　者：陈亚琳[1] 姜丹丹[2] 刘相萍[3] 王新刚[2] 李福年[2] 

机构地区：[1]青岛大学医学院,266071 [2]青岛大学医学院附属医院乳腺外科 [3]青岛大学医学院附属医院中心实验室

出　　处：《中华医学杂志》2013年第18期1407-1410,共4页National Medical Journal of China

基　　金：山东省自然科学基金（Y2007C134）

摘　　要：目的探讨基底细胞样乳腺癌细胞HCC1937中p21^wafl抗凋亡机制。方法采用RNAi技术干扰HCC1937乳腺癌细胞中p21^wafl基因表达，以未做处理的HCC1937为对照1组，感染空白载体慢病毒的HCC1937为对照2组，感染p21^wafl-shRNA慢病毒HCC1937为实验组。RT-PCR、Western印迹检测各组p21^wafl、bim基因的mRNA及蛋白表达。原位末端转移酶标记技术（TUNEL法）检测各组的细胞凋亡。结果实验组p21^wafl基因mRNA及蛋白表达量分别为0．260±0．004、0．293±0．006，对照1组分别为0．879±0．028、0．483±0．071，对照2组分别为0．870±0．025、0．469±0．047，与两对照组相比，实验组p21^wafl基因mRNA及蛋白表达量均显著降低，均P〈0．01。实验组bim基因mRNA及蛋白为0．420±0．013、0．355±0．007，对照1组分别为0．258±0．005、0．142±0．012，对照2组分别为0．259±0．002、0．147±0．013，与两对照组相比，实验组bim基因mRNA及蛋白表达量均显著增高，均P〈0．001。实验组细胞凋亡系数为0．279±0．012，对照1组为0．145±0．008，对照组2为0．148±0．012，均P〈0．001。结论在基底细胞样乳腺癌细胞HCC1937中，p21^wafl可通过抑制bim表达，发挥其抗线粒体凋亡功能。Objective To explore the anti-apoptotic mechanism of p21^wafl in human basal like breast cancer cell line HCC1937. Methods There were 3 groups, i.e. experimental group HCC1937 with lentivirus -p21^wafl -shRNA-RFP, control group 1 HCC1937 without lentivirus and control group 2 HCC1937 with lentivirus -RFP. The p21^wafl and bim mRNA and protein expressions were detected by reverse transcription-polymerase chain reaction （RT-PCR） and Western blot respectively. And apoptosis of HCC1937 in different groups was assayed by terminal deoxynucleotidyl transferase mediated C-dUTP nick end labeling （TUNEL）. Results After interference with letivirus-p21^wafl-shRNA-RFP, p21^wafl mRNA and protein expressions declined significantly in the experimental group versus the control groups （ experiment group： 0. 260 ±0. 004, 0. 293 ±0. 006, control group 1 ： 0. 879 ±0. 028, 0. 483 ±0. 071, control group 2： 0. 870 ± 0. 025, 0. 469 ± 0. 047, all P 〈 0. 01 ）. The bim mRNA and protein expressions increased. And there was significant difference between the experiment and control groups （experiment group： 0. 420 ± O. 013, 0. 355 ±0. 007, control group 1 ： 0. 258 ±0. 005, 0. 142 ±0. 012, control group 2： O. 259 ±0. 002, 0. 147 ± 0. 013, all P 〈 0. 001 ） ; apoptotic index increased （ experiment group： 0. 279 ± 0. 012, control group 1 ： 0. 145 ± 0. 008, control group 2 ： 0. 148 ± 0. 012, both P 〈 0. 001）. Conclusion In human basal- like breast cancer cell line HCC1937, p21^wafl exerts anti-apoptotic activity by inhibiting the expression of bim, a mediator of mitochondrial apoptosis.

关 键 词：乳腺肿瘤 P21 Bim 细胞凋亡 

分 类 号：R737[医药卫生—肿瘤]