牛蜘蛛腿综合征两个致病位点检测方法的建立  

Establishment of the detection method for two causative genes of cattle arachnomelia syndrome

作　　者：初芹[1] 焦士会[2] 王雅春[2] 刘林[3] 刘爱荣 吴宏军 谢振全 侯诗宇 耿繁军 汪聪勇 黄锡霞[8] 谭世新 谈锐 张毅[2] 俞英[2] 张沅[2] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100097 [2]中国农业大学动物科技学院,畜禽育种国家工程实验室,北京100193 [3]北京奶牛中心,北京100192 [4]内蒙古呼伦贝尔市海拉尔农垦集团有限责任公司,呼伦贝尔021008 [5]内蒙古谢尔塔拉种牛场,呼伦贝尔021008 [6]鞍山恒利奶牛场,鞍山114200 [7]河南省鼎元种牛育种有限公司,郑州450046 [8]新疆农业大学动物科学学院,乌鲁木齐830052 [9]天山畜牧生物工程有限公司,昌吉831109 [10]新疆维吾尔自治区畜牧总站,乌鲁木齐830009

出　　处：《遗传》2013年第5期623-627,共5页Hereditas（Beijing)

基　　金：国家科技攻关计划(编号:2011BAD28B02);现代农业产业技术体系专项资金(编号:CARS-37);长江学者与创新团队发展计划(编号:IRT1191)资助

摘　　要：牛蜘蛛腿综合征(Arachnomelia syndrome,AS)是一种隐性遗传疾病,虽然在瑞士褐牛和西门塔尔牛中症状相同,然而却是由两个不同的基因突变引起的,分别是SUOX基因c.363-364insG突变和MOCS1基因c.1224_1225delCA突变。文章利用51头西门塔尔牛及80头与配母牛和106头杂交后代,以及55头新疆褐牛公牛为研究群体,通过荧光标记引物PCR扩增结合毛细管电泳技术,建立了一种荧光自动化检测方法,能够快速、准确、一次性实现对引起牛AS疾病的两个突变位点的同时检测,为今后我国牛群中AS致病位点的筛查工作奠定了基础。Arachnomelia syndrome （AS） is a recessive inherited disease in cattle. Although the arachnomelia phenotypes are virtually identical in Brown Swiss and Sirnmental cattle, the causative mutation are different, which are a 1 bp insertion c.363-364insG in the sulfite oxidase （SUOX） gene and a 2 bp deletion c.1224 1225delCA in the molybdenum cofactor synthesis step 1 （MOCS1） gene, respectively. In the current study, combining fluorescence PCR with capillary electrophoresis technology, an automatic fluorescence method was established, which could detect the two causative loci rapidly and correctly with a single reaction. Samples from 51 Simmental bulls, 80 cows mated artificially using semen of Simmental bulls and their resulted 106 progeny, together with 55 Xinjiang Brown were collected and used for validation of the newly designed methods. Our results have laid a foundation for screening AS disease causing mutations in Chinese cattle.

关 键 词：蜘蛛腿综合征 西门塔尔牛 褐牛 SULFITE OXIDASE gene(SUOX) MOLYBDENUM COFACTOR synthesis STEP 1 gene(MOCS1) 

分 类 号：S858.23[农业科学—临床兽医学]