c-Src在TAM治疗耐药的人乳腺癌MCF-7细胞株中的表达及其意义  

Expression and significance of c-Src in human breast cancer MCF-7 cells resistant to tamoxifen therapy

作　　者：胡浩[1] 顾元龙[1] 朱从元[1] 黄阿雷[1] 周燕娜[1] 李建平[1] 

机构地区：[1]江苏省无锡市第三人民医院普通外科,江苏无锡214041

出　　处：《中国普通外科杂志》2013年第5期629-632,共4页China Journal of General Surgery

基　　金：江苏省无锡市卫生局科研资助项目(XM1010)

摘　　要：目的:探讨c-Src在乳腺癌三苯氧胺(TAM)中耐药的表达及意义。方法:用Western blot法检测TAM治疗耐药与敏感的两种人乳腺癌MCF-7细胞中c-Src的表达及其磷酸化水平;用c-Src阻滞剂PP2处理两种细胞后,采用酶联免疫吸附试验(ELISA)及吖啶橙/溴化乙锭(AO/EB)双荧光染色法检测细胞凋亡情况。结果:与TAM敏感MCF-7细胞比较,TAM耐药MCF-7细胞c-Src的表达量及其磷酸化水平均明显增高;PP2处理后,TAM敏感MCF-7细胞的富集指数(EF)及细胞凋亡率高于TAM耐药MCF-7细胞(均P<0.05)。结论:c-Src在TAM治疗耐药的人乳腺癌MCF-7细胞株中处于高表达及高活性状态,并可能参与乳腺癌内分泌治疗耐药的机制。Objective： To investigate c-Src expression in breast cancer resistant to tamoxifen（TAM） therapy and its signi cance.Methods： The expression and phosphorylation level of c-Src in TAM-resistant and sensitive human breast cancer MCF-7 cells were detected by Western blot analysis.e apoptosis in these two cell lines was detected by enzyme-linked immunosorbent assay（ELISA） and acridine orange/ethidium bromide（AO/EB） double uorescent staining a er treatment with c-Src inhibitor PP2.Results： Compared with TAM-sensitive MCF-7 cells,the levels of c-Src expression and phosphorylation inTAM-resistant MCF-7 cells were signiicantly increased.Ater exposure to PP2,the enrichment factor and cell apoptotic rate in TAM-resistant MCF-7 cells were signiicantly higher than those in TAM-sensitive MCF-7 cells（both P0.05）.Conclusion： here is a higher c-Src expression and activity in human breast cancer MCF-7 cell lines resistant to TAM therapy,which may probably be associated with the mechanism of endocrinotherapy resistance of breast cancer.

关 键 词：乳腺肿瘤 原癌基因蛋白质pp60(c-Src) 他莫昔芬 抗药性 肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]