鳀鱼蛋白酶水解物调血脂作用研究  

The antihyperlipermia effect of engraulis japonicus enzymatic hydrolysate in SD rats

作　　者：沈先荣[1] 林梦[2] 何颖[1] 蒋定文[1] 陈伟[1] 钱甜甜[1] 刘玉明[1] 李珂娴[1] 

机构地区：[1]海军医学研究所,上海200433 [2]浙江中医药大学,浙江杭州310053

出　　处：《中国海洋药物》2013年第3期33-39,共7页Chinese Journal of Marine Drugs

基　　金：全军医学科技"十二五"重大项目(AWS11J003)资助

摘　　要：目的研究鳀鱼(Engraulis)蛋白酶水解物调血脂功能。方法以鳀鱼为原料通过酶解法获得鳀鱼蛋白酶水解物。采用高血脂SD大鼠模型测定EJEH的调节血脂作用,建模后给药35d,测定大鼠血脂相关指标及SOD活性、MDA含量,进行肝组织病理形态学观察。结果与模型组比较,0.8、2.3和6.9g/kg.d-13个剂量EJEH组TG水平显著下降(P<0.05,P<0.01),2.3和6.9g/kg.d-1 EJEH组TC水平显著下降(P<0.05,P<0.01),6.9g/kg.d-1 EJEH组LDL-C水平显著下降(P<0.05);提示EJEH能显著降低高脂血症大鼠的血清TC、TG、LDL-C含量。病理学观察显示,各剂量组肝组织脂肪变性均有不同程度的改善。结论鳀鱼蛋白酶水解物具有明显的调血脂及改善肝脏脂肪变性功能。Objective To study the antihyperlipermia effect of Engraulis Japonicus enzymatic hydrolysate （EJEH） in SD rats. Methods EJEH was prepared from Engraulis Japonicus by enzymatic hydrolysis with 0. 05% alkaline proteinase and 0. 05% trypsin for 10 hours, and 0. 1% Flavored Proteinase for 2 h. The antihyperlipermia effect of EJEH was studied in SD rats. The EJEH were given daily for 35 days, and the model group and the control group were given with distilled water. At the 35th day, ser- um TC, TG, HDL-C, LDL-C, LDL-C/HDL-C, TG/HDL-C, SOD, MDA, liver weight, and micro- scope pathomorphism were tested. Results The level of the serum TC in the control group was 1. 359- 0. 147 mmol · L 1,in the model group was 1. 590-±-0. 056 mmol · L-1 （P〈0. 01, comparing with the control group）, in the 70 mg/kg d 1Xuezhikang positive control group was 1. 485±0. 102 mmol · L-1 （P（0. 05, comparing with the model group）, in the 2.3 g/kg· d-1 EJEH group was 1. 477±0. 098 mmol · L I（P〈0.05）, and in the 6.9 g/kg · d-IEJEH group was 1. 454±0. 052 mmol · L-1 （P〈0. 01）. The level of serum TG in the model group was 1. 480±0. 260 mmol · L-1 , which was increased significantly comparing with the control group （P〈0. 05）, and in the three EJEH treated groups the serum TG were decreased significantly comparing with the model group （P〈0.05, P〈0. 01）. The lev- el of serum LDL-C in the model group was 0. 960±0. 090, which was increased significantly comparing with the control group （P〈0.05）, and in the 6.9 g/kg · d ~EJEH group was 0. 849±0. 054 mmol · L （P〈0. 05, comparing with the model group）. The results suggested that EJEH decreased the ser- um levels of TC, TG, and LDL-C significantly. In the 6.9g/kg·d-IEJEH group, the serum SOD ac- tivity was increased significantly comparing with the model group （P〈0.05）. The liver adipose degen- eration in the Xuezhikang positive control group and the EJEH treated groups were ameliorated nota- bly. Conclusions EJEH has significant Anti

关 键 词：鳀鱼 蛋白酶水解物 血脂 高血脂症 肝脂肪变性 

分 类 号：R965[医药卫生—药理学]