基质金属蛋白酶及其组织抑制物预测葡萄胎恶变的研究  

Prediction of Malignant Transformation of Hydatidiform Mole by mRNA Determination of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinases

作　　者：李志英[1] 向阳[2] 戴理[3] 程国钧[4] 张德永[2] 杨秀玉[2] 宋鸿钊[2] 

机构地区：[1]湖北三峡学院医学院妇产科 [2]中国医学科学院中国协和医科大学北京协和医院妇产科,100730 [3]中国医学科学院基础医学研究所遗传室 [4]解放军总医院妇产科

出　　处：《中华妇产科杂志》2000年第9期547-550,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：卫生部科研基金资助(基金编号:981038)

摘　　要：目的　探讨基质金属蛋白酶 (MMP 9、MMP 2 )及其组织抑制物 (TIMP 1、TIMP 2 )表达量与葡萄胎恶变的相关性。方法　提取组织总RNA ,应用逆转录 聚合酶链反应 (RT PCR)技术检测2 2例正常早期妊娠绒毛及 37例葡萄胎组织中MMP 9、MMP 2、TIMP 1、TIMP 2基因表达量。结果　MMP 9、MMP 2、TIMP 1及TIMP 2在葡萄胎组织及正常绒毛组织中的表达量差异无显著性 (P >0 .0 5 )。但MMP 9/TIMP 1比值在发生恶变的葡萄胎组织明显高于未发生恶变的葡萄胎组织及正常绒毛组织(P <0 .0 1) ;而MMP 2 /TIMP 2在各组织中则无明显差异。结论　MMP 9/TIMP 1与葡萄胎恶变有关 ;MMP 9与TIMPObjective To investigate the relationship between the messenger RNA(mRNA) levels of matrix metalloproteinases(MMP 9, MMP 2) and tissue inhibitor of metalloproteinase (TIMP 1,TIMP 2) and malignant transformation of hydatidiform mole. Methods Total RNA were isolated from tissues of 22 normal chorionic villi samples and 37 cases of hydatidiform mole. The mRNA expression of MMP 9, MMP 2,TIMP 1, TIMP 2 genes were determined by RT PCR. Results The differences of the mRNA expression of MMP 9, MMP 2, TIMP 1, TIMP 2 genes in the tissues of hydatidiform mole and normal preganacy villus were not significant ( P >0.05). The ratio of MMP 9/TIMP 1 in molar tissues with malignant transformation was higher than those in molar tissues without malignant transformation and in normal villus. Conclusion It is a correlative relation beween MMP 9/TIMP 1 and malignancy of hydatidiform mole. The ratio of MMP 9/TIMP 1 might be used as an index for prediction of malignant transformation of hydatidiform mole.

关 键 词：金属蛋白酶类 金属蛋白酶类组织抑制剂 葡萄胎 

分 类 号：R737.33[医药卫生—肿瘤]