不同Cd^(2+)浓度与硝酸盐浓度、磷酸盐浓度交互作用对杜氏盐藻生长及叶绿素荧光特性的影响  

The interaction effects of Cd^(2+),nitriate and phosphate concentrations on the growth and chlorophyll fluorescence charteristics of Dunaliella salina

作　　者：王帅[1,2] 梁英[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]国家海洋局第一海洋研究所海洋生态中心,山东青岛266061

出　　处：《环境污染与防治》2013年第5期24-30,共7页Environmental Pollution & Control

基　　金：国家"863计划"项目(No.2007AA092001-10);国家科技支撑计划项目(No.2013BAK12B05);中央级公益性科研院所基本科研业务项目(No.2012G32);国家海洋局青年海洋科学基金资助项目(No.2012131);中国科学院实验海洋生物学重点实验室资助项目(KF2012No3)

摘　　要：研究了不同Cd2+浓度与硝酸盐浓度、磷酸盐浓度交互作用对杜氏盐藻(Dunaliella salina)生长及叶绿素荧光特性的影响。结果表明,Cd2+浓度与硝酸盐浓度、磷酸盐浓度对杜氏盐藻的光合作用及生长均具有显著影响(P<0.05);Cd2+胁迫24h后,杜氏盐藻的荧光参数Fv/Fm、Fv/Fo、Yield、rETR、细胞密度和叶绿素相对含量均与Cd2+浓度表现为显著的负相关;在低硝酸盐摩尔浓度(55μmol/L)下,杜氏盐藻的荧光参数Fv/Fm、Fv/Fo、Yield、rETR、细胞密度和叶绿素相对含量均明显下降;在高硝酸盐摩尔浓度(7 040μmol/L)下,杜氏盐藻的上述荧光参数下降幅度小;在正常硝酸盐摩尔浓度(880μmol/L)下,杜氏盐藻荧光参数Fv/Fo、NPQ和叶绿素相对含量均最高,对Cd2+的耐受性更强;在低磷酸盐摩尔浓度(2.27μmol/L)下,杜氏盐藻的荧光参数(除NPQ外)、细胞密度和叶绿素相对含量均显著低于对照组和高磷处理组,表现为磷限制现象;在高磷酸盐摩尔浓度(290.40μmol/L)下,杜氏盐藻的荧光参数(除NPQ外)、细胞密度和叶绿素相对含量均明显高于低磷处理组和对照组,杜氏盐藻受Cd2+胁迫程度最小。The interaction effects of Cd2＋ , nitriate and phosphate concentration on the growth and chlorophyll fluorescence charteristics of Dunaliella salina were investigated in this paper. The results indicated that Cd2＋ , nitri- ate and phosphate concentration had significant effects on the photosynthesis and growth of Dunaliella salina （P〈0.05）. The fluorescence parameters of Fv/Fm, Fv/Fo, Yield and rETR, cell density and relative chlorophyll content of Dunaliella salina were significant negative correlated with Cd2＋ concentrations after 24 h. And the fluorescence pa~ rameters evidently decreased in Dunaliella salina under low nitriate concentration of 55 μmol/L, and it also caused the decrease of the cell density and relative chlorophyll content. Under high nitriate concentration （7 040 μmol/L）, there was no significant difference in the chlorophyll fluorescence parameters. The fluorescence parameters （Fv/Fo, NPQ）, cell density and relative chlorophyll content of Dunaliella salina got to the greatest values at normal nitriate concentration of 880 μmol/L, and the Dunaliella salina had more tolerance to Cd2＋. The fluorescence parameters （except NPQ）, cell density and relative chlorophyll content of Dunaliella salina remarkably decreased in Dunaliella salina under low phosphate concentration （2.27 μmol/L）, which showed obvious phosphorus limitation. The chloro- phyll fluorescence parameters, cell density and relative chlorophyll content reached the highest values at high phos- phate of 290.40 μmol/L, and the Cd2＋ stress to Dunaliella salina was lowest,

关 键 词：杜氏盐藻 Cd2+ 硝酸盐浓度 磷酸盐浓度 叶绿素荧光 

分 类 号：X173[环境科学与工程—环境科学]