腺病毒转染对树突状细胞成熟的影响  

Effects of Recombinant Adenovirus Transfection on Maturation of Dentritic Cells

作　　者：崔慧霞[1,2] 李妍[2] 祁馨卉[2] 马楠[2] 姜又红[2] 张文陆[3] 

机构地区：[1]辽宁医学院护理学院基护教研室,辽宁锦州121001 [2]中国医科大学附属第一医院肿瘤研究所二室,沈阳110001 [3]辽宁医学院附属第一医院放疗科,辽宁锦州121001

出　　处：《中国医科大学学报》2013年第5期412-415,共4页Journal of China Medical University

基　　金：辽宁省科技厅社会发展攻关计划(2011415052-3);辽宁医学院青年基金(Y2012Z013)

摘　　要：目的研究腺病毒转染能否促进树突状细胞(DC)的成熟。方法分别用复制缺陷型腺病毒空载体(Ad-null)和表达乳腺珠蛋白的重组腺病毒(Ad-MGBA)转染DC(Ad-null DC组和Ad-MGBA DC组),用添加和不添加肿瘤坏死因子(TNF-α)的DC作为对照组(TNF-αDC组),另设DC空白对照组。用相差显微镜观察DC的形态变化,用流式细胞术检测各组DC表面成熟标志CD80、CD83、CD86的变化,用ELISA检测各组DC上清中白细胞介素12(IL-12)的变化。结果除DC空白对照组外,其余3组DC均表现出成熟DC的形态;转染DC组(Ad-null DC组和Ad-MGBA DC组)CD80、CD83、CD86、IL-12的表达水平均介于DC空白对照组和TNF-αDC组之间,在Ad-null DC组与Ad-MGBA DC组之间上述分子表达无统计学差异。结论转染腺病毒载体能够促进DC的成熟,但促进DC成熟的作用低于TNF-α对DC促成熟的作用。Objective To investigate the effects of recombinant replication-deficient adenovirus transfecfion on maturation of dentritie cells （DC）. Methods DC were transfeeted with recombinant replication-deficient null adenovirus （Ad-null） and recombinant adenovirus encod- ing mammaglobin A （Ad-MGBA）, respectively. Non-transfected DC and DC treated by TNF-α were used as controls. The morphological char- acteristics of DC were observed under a phase-contrast microscope. The cell surface expressions of CD80 ,CD83, and CD86 on DC were measured by flow cytometry （FCM）. The level of IL-12 was detected by EL1SA. Results Except for non-transfected DC, all the other three DC groups displayed maturation characters. The levels of CD80,CD83,CD86 and IL-12 on DC transfected with Ad-null and Ad-MGBA were between those of non-trausfected DC and TNF-α treated DC. Conclusion Transfection with adenovirus alone can induce DC maturation, however, the stimulation effect is lower than TNF-α induction.

关 键 词：重组腺病毒 树突状细胞 转染 

分 类 号：R730.51[医药卫生—肿瘤]