诱发肝癌肝细胞病理改变与^(125)I-胰岛素摄取率的动态观察  

Dynamic Study of Hepatocellular Pathological Change and Uptak e Rate of ^(125)I insulin During Experimental Hepatocarcinogenesis

作　　者：匡安仁[1] 梁正路[1] 谭天秩[1] 王朝俊[2] 江映红[2] 杨可 

机构地区：[1]华西医科大学附属第一医院核医学科,成都610041 [2]华西医科大学附属第一医院肿瘤研究所

出　　处：《华西医科大学学报》2000年第3期289-291,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金!(批准号 39770229)

摘　　要：为探索肝细胞癌变导致摄取125I－胰岛素增加的可能机理，用二乙基亚硝胺（DENA）诱发大鼠原发性肝细胞癌，动态观察癌变过程中大鼠肝脏浓聚125I－胰岛素能力增强与肝细胞病理改变之间的关系。将SD大鼠80只随机分为实验组（n=40）和对照组（n=40）。两组均饲以普通饲料，实验组按70mg/kg的剂量每周1次灌胃给予DENA。实验第6、11、15和20周，由尾静脉注射125I胰岛素溶液后1小时断头处死对照组和实验组的大鼠各10只，收集血、肝、肺、脾、肾、肌和骨，称重并测量放射性，计算% ID/g。肝组织作病理组织学检查。结果显示：所有对照组大鼠肝脏组织学观察均正常。第6周实验组肝脏表面粗糙，镜下发现肝细胞增生活跃。实验组125I－胰岛素摄取率是对照组的1.86倍；第11周肝脏颜色变浅，边缘稍钝，其中1只大鼠的肝脏呈弥漫性细小颗粒，病检表现为重度增生和肝硬变，125I－胰岛素摄取率是对照组的1.50倍；第15周，除以上的病理改变外，6只实验大鼠肝脏镜下可见肝癌细胞，125I－胰岛素摄取率是对照组的1.56倍；第20周，所有实验组的肝脏长大变形、质硬、弥漫性大小不等的灰白色结节，有坏死、液化和出血，10只实验组大鼠肝脏均发现肝癌细胞，125I－胰岛素摄取率是对照组的1.46倍。This study was designed to observe the hepatocellular pathological change and uptake rate of 125I－insulin during experimental hepatocarcinogenesis in rats and clarify the possible mechanism of increasing up take of 125I－insulin. 80 SD rats were divided into 2 groups, control and experi mental groups. All of the rats were given common feed, and the rats of experimen tal group were given extra diethylnitrosamine(DENA) 70mg/kg each week. At 6th, 1 1th, 15th and 20th week after start of the experiment, 10 control and 10 experi mental rats were killed 1 hour post administration of 125I－insulin by ta il vein. Blood, liver, lung, spleen, kidney, muscle and bone were collected; the radioactivity was measured and calculated %ID/g. The data of 2 groups were comp ared and examined with t －test. All of livers were pathologically examined. The results showed all livers of control group were normal. At 6th week, the su rfaces of experimental rats' livers were coarse. Hepatocellular hyperplasia was observed,125I－insulin uptake rate was 1.86 time as much as that of contr ol group. At 11th week, the liver's colour became lighter than that of control g roup. Hyperplasia and hepatocirrhosis were observed,125I insulin uptake rate was 1.50 time as much as that of control group. At 15th week, hyperplastic nodules were observed in all experimental rats' livers. Hepatomacellulae were ob served in 6 rat livers.125I insulin uptake rate was 1.56 time as much as that of control group. At 20th week, the livers became enlarged out of shape. T here were a lot of big or small greyish white nodules in all livers. Necrosis, l iquefaction and hemorrhage were observed. Hepatomacellulae were observed in all of experimental rats livers.125I insulin uptake rate was 1.46 time as mu ch as that of control group. The differences of 125I insulin uptake rat e between experimental and control groups were significant. These results demons trated that the liver ability of uptaking 125I insulin increased which m ainly too

关 键 词：肝癌 受体 肝细胞癌 ^125I-胰岛素 摄取力 DENA 

分 类 号：R735.7[医药卫生—肿瘤]