大鼠视交叉上核内主要的三种肽能神经元的分布  

Distribution of Peptidergic Neurons in the Rat Suprachiasmatic Nucleus

作　　者：王蕾[1] 欧可群[1] 操高原[1] 陈文玉[1] 

机构地区：[1]华西医科大学基础医学院组织学与胚胎学教研室,成都610041

出　　处：《华西医科大学学报》2000年第3期322-324,369,共4页Journal of West China University of Medical Sciences

基　　金：高等学校博士学科点专项基金

摘　　要：研究视交叉上核内主要肽能神经元的分布情况，选用健康成年雄性SD大鼠5只，以免疫组化双重及三重标记技术，对大鼠视交叉上核(SCN)内血管活性肠多肽（VIP）、精氨酸血管加压素（AVP）及生长抑素（SOM）样三类神经细胞群的分布进行了观察。免疫染色以ABC法进行,呈色底物采用DAB、TMB及4－氯－1－萘酚。结果显示：在SCN头中尾三段,三种肽能神经元的数量及分布均存在差异；VIP与AVP样细胞群之间并不是截然分界，其相交处一狭窄区内三种肽能神经元混杂在一起，交错重叠。上述发现为SCN机能学的研究提供了新的形态学依据。The distribution of the peptidergic neurons in t he suprachiasmatic nucleus(SCN) in five adult male SD rats was examined by multiimmunolabellingfor vasoactive intestinal polypeptide(VIP),arginine vasopressin(AVP) and somatostatin(SOM).The immunoreactions were visualized with an Elite ABC Kit(Vector Lab) . The substrates in the peroxidase reaction were diaminobenzidine(DAB), tetramet hylbenzidine(TMB) and 4－chloro－1－naphtol. The results showed that the differe nces in distribution and population of VIP－,AVP－ and SOM－like neurons existed in the rostral,intermediate and caudal regions of the SCN and these neurons were over lapped and SOM－like neurons were scattered among them in the area between VIP and AVP－like neurons. These findings may provide new morphologic evidences for explaining the functional mechanism in the SCN.

关 键 词：视交叉上核 VIP AVP 生长抑素 昼夜节律 

分 类 号：R339.146[医药卫生—人体生理学] Q811.213[医药卫生—基础医学]