川芎嗪对脑缺血再灌注时细胞凋亡的影响  

The effects of ligustrazini on apoptosis after focal cerebral ischemia followed by reperfusion

作　　者：吕少平[1] 孙锋[1] 王春霞[1] 

机构地区：[1]青岛医学院第二附属医院神经科,山东青岛266042

出　　处：《河北医学》2000年第8期673-675,共3页Hebei Medicine

摘　　要：目的 :探讨川芎嗪对脑缺血再灌注时细胞凋亡的影响。方法 :对实验大鼠于脑缺血前应用川芎嗪进行干预 ,然后采用免疫组化法检测脑缺血再灌注不同时间内凋亡相关基因 c- fos及 bcl- 2蛋白表达的变化。结果 :川芎嗪组大鼠脑缺血后的梗死体积显著减小 ,川芎嗪组脑缺血再灌注时皮层和基底节区 c- fos的表达明显下降 ,而 bcl- 2的表达明显增高。结论 :川芎嗪可抑制脑缺血再灌注时细胞凋亡的发生 。ObjectiveTo investigate the effect of Ligustrazini(LZ)on apoptosis after focal cerebral ischemia followed by reperfusion.Methods:Rats were pretreated with LZ prior to MCAO,changes of protein expression of cfos and bcl2 in rats after ischemia and reperfusion were observed by immunohistochemical method.Results:The infarct volume in LZ group was significantly smaller than that in the corresponding regions in the control group.In LZ group ,the protine expression of cfos decreased significantly after ischeima/reperfusion both in the ischemic cortex and in the ischemic basal ganglia ,but the protein expression of bcl2 increased significantly after ischemia/reperfusion both in the ischemic ocrtex and in the ischemic basal ganglia.Conclusions:LZ may inhibit the development of apoptosis in rats after ischemia and reperfusion and have the neuroprotective effect against ischemia.

关 键 词：脑缺血 C-FOS BCL-2 川芎嗪 细胞凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]