响应曲面法优化红菜苔多酚提取工艺的研究  

Optimization of Extraction Process of Polyphenols from Brassica campestris L. var. purpurea Baileysh by Response Surface Analysis(RSA)

作　　者：杨红 操德群 朱江 谢顺岚 李建华 

机构地区：[1]特色果蔬质量安全控制湖北省重点实验室,湖北孝感432000

出　　处：《食品研究与开发》2013年第8期5-9,共5页Food Research and Development

基　　金：国家级大学生创新创业训练计划项目资助(201210528001)

摘　　要：利用中心组合设计探讨提取的温度、时间、乙醇浓度和液固比这4个因素对红菜苔叶和茎皮中多酚得率的影响,构建具有良好预测性能的数学模型,通过响应曲面分析法确定红菜苔叶和茎皮中多酚最优提取工艺条件。试验结果表明,提取温度、乙醇浓度和液固比对红菜苔叶和茎皮中多酚得率有极显著的影响(P<0.01);二次方模型为本研究的最适模型;最优提取条件为:提取温度59.68℃,提取时间2.67 h,乙醇浓度64.60%,液固比53.57∶1(mL/g),最优提取量是1.529 3 mg/g。A central composite design was used to investigate the effects of four factors just as extraction temperature, extraction time, ethanol concentration and liquid-solid ration on the yield of polyphenols from Brassica campestris L. leaves and stem barks. A predictability mathematical model was constructed. The optimum conditions for the ethanol extraction of polyphenols were determined using response surface analysis （RSA）. The results showed that extraction temperature, ethanol concentration and liquid-solid ration had significant influence on the yield of polyphenols from Brassica campestris L. leaves and stem barks. The quadratic mathematical model was the optimal model for this study. And the optimum extraction were as follow： extraction temperature was 59.68℃, extraction time was 2.67 hours, ethanol concentration was 64.60 %, liquid-solid ration was 53.57 ： 1 （mL/g）, and the optimal yield was 1.529 3 mg/g.

关 键 词：红菜苔 多酚 响应曲面分析 

分 类 号：TQ914[化学工程]