正常人脑Glob-N的分离及其结构的初步分析  

Isolation and preliminary structural characterization of Glob-N from normal human brain

作　　者：张宗城[1] 张积仁[1] 张健[1] 林永成[2] 汪森明[1] 

机构地区：[1]第一军医大学珠江医院肿瘤中心,广东广州510282 [2]中山大学化学系天然有机研究室,广东广州510275

出　　处：《第一军医大学学报》2000年第4期382-384,共3页Journal of First Military Medical University

基　　金：国家自然科学基金资助课题(39700143)

摘　　要：目的 探索一种新的脑胶质瘤生长抑制因子Glob-N的分离鉴定方法。方法 人脑中性鞘糖脂经过加压柱层析，梯度甲醇/氯仿溶液洗脱。对所得到的产物行1H-NMR和13C-NMR测定。结果 含甲醇60%的甲醇/氯仿溶液的洗脱物为纯化Glob-N，层析展开后呈单一清晰条带，Rf值0.45。初步结构分析表明人脑Glob-N含N-乙酰半乳糖，共3个糖基，其神经酰胺部分共有33个碳原子。结论 柱层析分离中性鞘糖脂单一组分效果好，纯度高；人脑Glob结构中含3个糖基。Objective To develop a new method for isolating and identifying a glioma growth factor inhibitor, Glob-N, from normal human brain. Methods Neutral glycosylsphingolipid was separated from normal human brain by column chromatography with methanol/chloroform solution for gradient elution, and the resultant underwent 1H-NMR and 13C-NMR analyses for identifying the structure of Glob-N. Results Glob-N was obtained by elution with 60% (V/V) methanol in methanol/chloroform solution, with the Rf value of 0.45. The structural analysis showed that Glob-N from human brain was a globotriaosylceramide, containing GalNAc with 33 carbon atom in its ceramide moiety. Conclusion Individual N-GSL can be well separated from total N-GSL by column chromatography with high output. Glob-N from human brain maybe a kind of globotriaosylceramide.

关 键 词：柱层析 分离 纯化 脑胶质瘤 生长抑制因子 

分 类 号：R73-3[医药卫生—肿瘤]