小鼠B16黑色素瘤细胞HACs的提纯及其抑瘤作用和机理的探讨  

Tumor inhibiting effects and its mechanism of HACs purified from mice B16 melanoma cell

作　　者：付士波[1] 李秀娟[1] 孙祖玥[1] 龚守良[1] 李修义[1] 杨英[1] 

机构地区：[1]吉林省长春市白求恩医科大学卫生部放射生物重点实验室,吉林长春130021

出　　处：《癌症》2000年第9期900-902,共3页Chinese Journal of Cancer

摘　　要：目的：从小鼠B16黑色素瘤细胞胞浆中提纯热休克蛋白抗原肽复合物(heat shock protein-antigen peptide complexes,HACs)，并观察其抑瘤作用及探索其机制。方法：CNBr-Sepharose 4B亲和层析法提纯B16 HACs，应用体内免疫法检测HAC的抑瘤作用，结晶紫染色法检测γIFN分泌活性，ConA诱导的淋巴母细胞增殖法检测IL2分泌活性，3H-TdR掺入法检测特异性CTL的杀伤活性。结果：亲和层析法获得的HAC60、HAC75和HAC97免疫小鼠后，小鼠移植肿瘤的发生率降低，肿瘤平均出现时间延迟，平均肿瘤重量明显减轻；小鼠脾细胞的γ-IFN和IL-2分泌活性增强，特异性CTL的杀伤活性增强。结论：经亲和层析纯化的HACs可通过增强免疫功能的机制抑制小鼠移植肿瘤的生长，并为制备肿瘤疫苗提供实验依据。Objective： In the present study, we purified heat shock protein antigen peptide complexes (HACs) from mice B16 melanoma cytosol, and investigated its tumor inhibitory effect and mechanisms. Methods： Affinity chromatography was used to purify B16 HACs. γ IFN and IL 2 secreting activities were determined with crystal voilet staining and lymphoblast proliferation induced by Con A, respectively. Pecific CTL killing rate was detemined with 3H TdR incorporation. Results： Following immunization with HAC60, HAC75 and HAC97 obtained through affinity chromatography, the incidence of melanoma in the mice received B16 melanoma implantation decreased, the time of tumor development delayed and tumor weight reduced. While, γ IFN and IL 2 secreting activities of mouse spleen cells and specific CTL killing rates were all increased. Conclusion： HACs may inhibit tumor growth through mechanism of enhancing immune function, which offers the experiment evidence for preparing tumor vaccines.

关 键 词：黑色素瘤 热休克蛋白抗原肽复合物 亲和层析法 

分 类 号：R730.2[医药卫生—肿瘤]