TAMs在皮肤恶性黑素瘤组织中的浸润及与STAT3、VEGF蛋白表达的关系  

The Infiltration and Significance of Tumor Associated Macrophages in Cutaneous Malignant Melanoma Skin Tissue and the Relationship with STAT3 and VEGF Protien Expression

作　　者：殷芳[1] 吴飞[1] 陈佳[2] 章楚光[2] 王萍[3] 宋宁静[2] 

机构地区：[1]安徽医科大学上海皮肤病临床学院,上海市200443 [2]安徽医科大学上海皮肤病临床学院(上海市皮肤病医院)病理科,200443 [3]上海交通大学医学院附属第九人民医院病理科

出　　处：《中华全科医学》2013年第6期840-843,F0003,共5页Chinese Journal of General Practice

基　　金：上海市自然科学基金项目(11ZR1432900);上海市卫生局科研项目(20114125)

摘　　要：目的探讨皮肤恶性黑素瘤组织中TAMs浸润与STAT3、VEGF蛋白表达及患者临床病理特征的关系。方法采用免疫组化法检测40例CMM组织及15例色素痣组织中CD68(标记TAMs)及VEGF、STAT3蛋白的表达情况。结果 CMM组织TAMs计数为(29.6±9.1)个/HP,STAT3及VEGF蛋白中的阳性表达分别为70.0%(28/40)、62.5%(25/40);而色素痣组织分别为(10.5±3.8)个/HP、13.3%(2/15)、0%(0/15);两两比较,P值均<0.001。CMM组织中TAMs计数、STAT3及VEGF蛋白表达与Clark分级和有无淋巴结转移密切相关(P<0.001,P<0.001;P=0.007,P=0.004;P=0.008,P=0.004)。TAMs在STAT3蛋白及VEGF蛋白阳性组织中的计数分别显著高于在STAT3蛋白及VEGF蛋白阴性组织中的计数(P值均<0.001),STAT3蛋白表达与VEGF蛋白之间也具有显著相关性(P值<0.001)。结论 CMM组织中TAMs浸润与STAT3、VEGF蛋白的表达密切相关,且三者均与Clark分级和有无淋巴结转移有关;TAMs可能通过调节STAT3、VEGF蛋白的表达,对CMM的发生和发展起促进作用,有望成为CMM的靶向性治疗提供新思路。Objective To investigate the infiltration and significance of Tumor associated macrophages in cutaneous malig- nant melanoma skin tissue as well as the relationship with STAT3, VEGF protien expression and clinicopathologic feature in cuta- neous malignant melanoma tissue. Methods Immunohistochemistry was used to detect the expression of TAMs marked CD68, STAT3 ,VEGF protein expression in 40 cutaneous malignant melanoma tissue specimens and 15 nevus tissue specimens. Results In cutaneous malignant melanoma tissue, the TAMs count was 29.6 ±9.1/HP, STAT3 protien expression was positive in 28 cases（70.0% ） ,VEGF protein expression was positive in 25 cases（62.5% ）. But 10.5 ± 3.8/HP,2 cases（ 13.3% ） ,no positive case in nervus tissue. TAMs count, STAT3, VEGF protien expression were correlated with Clark grading and lymphatic metastasis in cutaneous malignant melanoma （ P 〈 0.001, P 〈 0. 001 ; P = 0＂. 007, P = 0.004 ; P = 0.008, P = 0.004 ）. TAMs count in STAT3 protein positive expression tissue was significantly higher than in negative expression tissue, so was TAMs count with VEGF protein expression（P 〈 0.01 ）. The expression of STAT3 protein was significant correlated with VEGF protein expression （ P 〈 0.01 ）. Conclusion The infiltration of TAMs was closely correlated with the expression of STAT3 and VEGF protein in cu- taneous malignant melanoma,they were also correlated with Clark grading and lymphatic metastasis in cutaneous malignant mela- noma. TAMs might play an important role in the development and progression of cutaneous malignant melanoma,which might be a new potential target for therapy of it.

关 键 词：TAMS STAT3 VEGF 皮肤恶性黑素瘤 

分 类 号：R730.21[医药卫生—肿瘤] R739.5[医药卫生—临床医学]