Survivin在小鼠胚胎植入过程中的动态变化  

Dynamic Changes of Survivin in Mouse Endometria During Implantation

作　　者：熊国平[1] 刘孝华[2] 汪琳[3] 

机构地区：[1]武汉市中心医院,湖北武汉430014 [2]湖北民族学院附属医院,湖北恩施445000 [3]武汉大学基础医学院,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2013年第3期348-351,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30772307)

摘　　要：目的:检测Survivin在围植入期小鼠子宫内膜组织中的表达,探讨其在胚胎植入过程中的作用。方法:选择成年雌性昆明小白鼠42只,阴道脱落细胞涂片法确定动情周期。将处于动情周期的雌鼠与性成熟雄鼠合笼后次晨检查阴道分泌物涂片,有精子者记为妊娠0 d。采用免疫组织化学和RT-PCR法分别检测妊娠0,3.5,4.5,5.5,6.5,7.5及9 d各组子宫内膜组织中Survivin蛋白及mRNA水平的变化。结果:①Survivin蛋白在妊娠0 d小鼠子宫内膜组织中表达较低,其阳性产物光密度(OD)值为0.058 1。之后逐渐增高,至妊娠5.5 d达峰值为0.140 7。之后逐渐下降,至妊娠9 d降至0.074。统计学检验结果提示:Survivin蛋白在胚胎植入期(4.5 d,5.5 d,6.5 d)表达较高,其OD值与胚胎植入前期(0 d,3.5 d)及胚胎植入后期(7.5 d,9 d)的差异均有显著性(P<0.05)。②Survivin mRNA的表达在妊娠0 d小鼠子宫内膜组织中较低,其与内参相比的相对表达量为0.330 4。之后逐渐增高,至妊娠6.5 d达峰值为1.310 7。之后逐渐下降,至妊娠9 d降至0.598。统计学检验结果提示:Survivin mR-NA在胚胎植入期表达较高,其OD值与胚胎植入前期及胚胎植入后期的差异均有显著性(P<0.05)。③Survivin蛋白及mRNA在胚胎植入后期小鼠子宫内膜组织中的表达略高于胚胎植入前期,但差异无显著性(P>0.05)。结论:Survivin在胚胎植入前期和后期均低表达,植入期高表达。这种变化提示Survivin是影响胚胎植入的重要因素之一。Objective： To profile Survivin expression pattern in mouse endometrium during embryonic implantation process.Methods： Forty-two pregnant mice were divided into seven groups according to different pregnant days（pregnant 0,3.5,4.5,5.5,6.5,7.5,and 9 days）.Immunohistochemical staining and RT-PCR were employed to detect the protein and mRNA expression of Survivin in mouse endometria.Results： ①The expression level of Survivin in mouse endometria during the embryo implantation prophase（pregnant 0-day,3.5 d） were significantly lower than that in embryonic implantation phase（4.5 d,5. 5 d,6.5 d）（P〈 0.05）.②The expression level of Survivin in endometria,after embryonic implantation completion（7.5 d,9 d）,were also significantly lower than that in embryo implantation phase（4.5 d, 5.5 d,6.5 d）（P〈 0.05）.③The expression level of Survivin in mouse endometria seems to be lower during embryonic implantation prophase than in embryonic implantation anaphase,but showed no significant difference（P〈 0.05）.Conclusion： The expression of Survivin in mouse uterus in the embryonic implantation prophase and the embryo implantation anaphase were lower than that in embryonic implantation phase.This change implied that Survivin might be an important factor to influence the embryonic implantation.

关 键 词：SURVIVIN 胚胎植入 子宫内膜 

分 类 号：R374.5[医药卫生—病原生物学]