曲古霉素A促进胶质瘤细胞系凋亡  

Trichomycin A facilities the apoptosis of glioma cell line

作　　者：马爽[1] 翟志勇[1] 郭阳[1] 

机构地区：[1]中国医科大学附属盛京医院神经内科,辽宁沈阳110004

出　　处：《解剖科学进展》2013年第3期282-285,289,共5页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金资助项目(No.2007211)

摘　　要：目的组蛋白的异常修饰(去乙酰化)与胶质瘤的发生和发展关系密切,本研究的目的是探讨组蛋白去乙酰化酶抑制剂曲古霉素A(trichomycin A,TSA)对胶质瘤细胞系凋亡的调节作用。方法将浓度分别为0.2μmol/L、0.4μmol/L和0.8μmol/L的TSA加入胶质瘤细胞系U251细胞中,应用Westernblot法检测U251细胞系经不同浓度的TSA处理后细胞内胱氨酸蛋白酶-3(caspase-3),乙酰化组蛋白H3和H4的表达,应用流式细胞术(Annexin V-FITC染色)检测不同药物浓度处理组中U251细胞的凋亡率。结果 TSA处理后,U251细胞内乙酰化组蛋白H3、H4及caspase-3的蛋白水平明显上调(P<0.01),具有明显的剂量依赖性。流式细胞术结果显示U251细胞TSA处理后细胞的凋亡率具有剂量依赖性显著上调。结论 TSA剂量依赖性上调胶质瘤细胞系U251中caspase-3表达,促进细胞凋亡。Objective Many studies have shown that abnormal modification of histones （deacetylation） closely correlated with the occurrence and development of glioma, the purpose of this study is to investigate the effect of trichomycin A （TSA） on the apoptosis of glioma cell line U251. Methods Different concentrations of TSA （0.2 μmol/L, 0.4μmol/L and 0.8μtool/L） were used to treat glioma cell line U251, respectively. Western blot was used to detect tile protein expression of easpase-3, aeetylated hisitone H3 and H4 in U251 cells after TSA treatment. Cell apoptosis rate was detected by flow eytometry using Annexin V-FITC method. Results TSA treatment up-regulated the caspase-3 protein level significantly with a manner of dose-dependent （P〈0.01）. Flow eytometry showed that the apoptosis rate of U251 cells were up-regulated significantly after TSA treatment with a dose-dependent manner. Conclusion TSA treatment could up-regulate easpase-3 expression and promote the apoptosis rate of glioma cell line U251 with a dose-dependent manner.

关 键 词：曲古霉素A 胶质瘤 凋亡 

分 类 号：R730.264[医药卫生—肿瘤]