FOS蛋白在胶质瘤中的表达及其对胶质瘤细胞生长的影响  

Expression of FOS protein in glioma and its effect on the growth of human glioma cells

作　　者：陶陶[1] 陆小明[1] 姚磊[1] 王佳甲[1] 史岩[1] 骆慧[1] 刘宁[1] 尤永平[1] 

机构地区：[1]南京医科大学第一附属医院神经外科,210029

出　　处：《中华医学遗传学杂志》2013年第3期293-296,共4页Chinese Journal of Medical Genetics

基　　金：基金项目：国家自然科学基金（81272792,81072078,81172389）;江苏省自然科学基金（BK2010580）;江苏省高校优势学科建设工程资助项目（JX10231801）;江苏省医学重点学科资助项目;南京医科大学第一附属医院创新团队工程

摘　　要：目的探讨FOS蛋白在胶质瘤中的表达及其对胶质瘤细胞生长的影响。方法用免疫组化染色法检测FOS蛋白在各级别胶质瘤组织中的表达水平。应用RNA干扰技术降低FOS表达后，应用四甲基偶氮唑盐微量酶反应比色法和流式细胞技术检测胶质瘤细胞的增殖和周期的改变；应用Transwell实验检测胶质瘤细胞侵袭能力的改变；采用Western印迹法检测FOS下游功能蛋白的变化。结果胶质瘤组织中FOS的表达随着级别的增高而增高；抑制FOS的表达后，U87和U251细胞生长受抑制，细胞生长阻滞在G1期且细胞侵袭能力下降，下游的功能蛋白CyclinD1和MMP9表达水平降低。结论FOS在胶质瘤中高表达；抑制其表达可以下调功能蛋白CyclinD1和MMP9的表达，进而调控细胞的生长和侵袭能力。Objective To study the expression of FOS protein in human glioma tissues and its effect on tumor growth. Methods FOS protein expression in glioma tissues was determined with immunohistochemical （ IHC ） staining. Subsequently, 3-（ 4, 5 ）-dimethylthiahiazo （-z-y1）-3, 5- diphenytetrazoliumromide （MTT） assay, flow cytometry, transwell invasion and Western blotting were used to assay U87 and U251 cells with reduced FOS expression. Results The expression of FOS in glioma was increased and strongly correlated with its pathological grade. Abrogating expression of FOS has suppressed proliferation and invasion, and delayed cell cycle at G1 phrase for both U87 and U251 cells. Conclusion The expression of FOS protein in human glioma was strong. FOS protein probably plays a critical role in the progression of gliomas.

关 键 词：胶质瘤 FOS蛋白 CYCLIND1蛋白 MMP9蛋白 细胞增殖 细胞周期 细胞侵袭 

分 类 号：R739.4[医药卫生—肿瘤]