左乙拉西坦对红藻氨酸致发育期癫痫幼鼠海马主穹窿蛋白表达的影响  

Effect of levetiracetam on the expression of major vault protein in developing rat hippocampus following kainic acid-induced seizure

作　　者：孙明霞[1] 戴园园[1] 李蕊[1] 张亚楠[1] 程华[1] 

机构地区：[1]徐州医学院附属医院儿科,徐州221002

出　　处：《神经解剖学杂志》2013年第3期280-284,共5页Chinese Journal of Neuroanatomy

摘　　要：目的:建立发育期慢性癫痫大鼠模型,观察海马主穹窿蛋白(MVP)的表达及左乙拉西坦(LEV)对其表达的影响。方法:腹腔注射红藻氨酸(KA)1 mg/kg(浓度0.5 mg/ml),建立大鼠慢性癫痫模型,注射后连续观察8 h,癫痫发作达5级以上并出现癫痫持续状态的大鼠,且两周后出现自发性反复惊厥发作者视为模型成功。将造模成功后的40只大鼠随机分为未治疗组(KA组)20只,左乙拉西坦治疗组(KA+LEV组)20只,另取40只大鼠腹腔注射生理盐水,并分为NS组20只、NS+LEV组20只。用药组均于癫痫自发性反复发作后开始用药,疗程为6周,然后将所有大鼠断头取脑,用免疫组化及RT-PCR法测定大鼠海马内MVP及其mRNA的表达。结果:(1)大鼠海马MVP的阳性细胞计数与MVP的mRNA表达趋势相一致。(2)NS组、NS+LEV组大鼠海马有少量MVP阳性细胞及mRNA表达,NS+LEV组MVP阳性细胞数及mRNA的含量与NS组相比差异无统计学意义(P>0.05);KA组MVP阳性细胞计数及mRNA的含量与NS组相比显著增高(P<0.05);KA+LEV组与KA组相比,MVP的阳性细胞及mRNA含量减少(P<0.05)。结论:MVP可能参与慢性癫痫耐药的发生,LEV可以控制大鼠痫性发作,并下调MVP的表达。Objective： To observe the expression of major vault protein （MVP） in the hippocampus of the chronic epileptic rats, and explore whether anticpileptie drugs levetiracetam （LEV） affect the expression of MVP. Methods： We established the rat model of chronic epilepsy by peritoneal injection of kainic acid （KA） 1 mg/kg （ concentration of 0.5 mg/ml）. Those rats who reached stage five seizures and became status epilepticus after 8 hours of peritoneal injection of KA were regarded as successful seizure models if they showed spontaneous recurrent seizure after two weeks. Such 40 rats of successful models were randomly divided into KA group （n = 20） and KA ＋ LEV group （n = 20）. The control rats only received an injection of same amount of saline, and they were randomly divided into NS group （ n = 20 ） and NS ＋ LEV group （ n = 20）. After spontaneous recurrent seizure, the treatment groups began to take drug, and the treatment time is 6 weeks. Immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR） methods were used respectively to detect MVP protein and mRNA expression after these rats＇ brains removed. Results： （ 1 ） MVP mRNA expression trends were consistent with the corresponding protein expression levels. （2） Lightly stained cells were sparsely observed in the NS group and NS ＋ LEV group. There was no significant difference in the number of MVP positive eells or the expression of MVP mRNA levels between these two groups （ P 〉 0.05 ）. The number of MVP positive cells and the expression of MVP mRNA levels in the KA group were significantly higher than those in the NS group （ P 〈 0.05 ）. As compared with KA group, the number of MVP positive cells and the mRNA levels in the KA ＋ LEV group were reduced （ P 〈 0.05）. Conclusion： MVP may play an important role in the resistance of chronic epilepsy, while LEV can control rats epileptic seizure, and reduce the MVP expression.

关 键 词：主穹窿蛋白 癫痫 红藻氨酸 发育期 左乙拉西坦 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]