创伤后应激障碍大鼠海马神经元自噬增强  

Enhancement of autophagy in hippocampus of rat with post-traumatic stress disorder

作　　者：隋竹欣[1] 刘昊[2] 朱玉英[1] 熊佩黎[1] 纪荣静[1] 王海涛[1] 

机构地区：[1]河北联合大学基础医学院组织胚胎学系,唐山063000 [2]河北联合大学附属医院神经内科,唐山063000

出　　处：《神经解剖学杂志》2013年第3期325-328,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81201048);河北省自然基金项目(H2012401009);河北联合大学大学生创新性实验计划(X2012028)

摘　　要：目的:观察创伤后应激障碍(PTSD)模型大鼠海马神经元自噬的改变,探讨PTSD致海马体积异常的可能机制。方法:成年健康雄性SD大鼠20只,随机被分为对照组和模型组。采用改良的单一连续应激后给予不可逃避足底电击方法制备PTSD大鼠模型;采用透射电镜技术观察海马神经元超微结构,Western blot方法检测海马微管相关蛋白轻链3(microtube-associated protein 1 light chain 3,LC-3)和Beclin-1的表达水平。结果:模型组海马神经元自噬体增多;海马组织LC3-Ⅱ/LC3-Ⅰ比值和Beclin-1表达水平高于对照组(P<0.05)。结论:PTSD模型大鼠海马神经元存在明显的细胞自噬,可能与海马体积异常变化有关。Objective： To observe the change of autophagy in hippocampal neurons of rat with post-straumatic stress disorder （PTSD）, and to investigate the possible mechanism of hippocampal volume alteration in PTSD. Methods： Twenty adult male SD rats were randomly divided into control group and PTSD model group. The PTSD animal model was established by using the modified single-prolonged stress followed by a single inescapable electric foot shock（ SPS ＆ S）. The uhrastructure of neurons in hiappoeampus was observed under electron microscope. The expression of LC-3 and Beclin-1 protein in hippocampus was detected by Western blot. Results： Autophagosomes increased in hippocampal neurons of PTSD model rats. The ratio of LC3-Ⅱ to LC3-Ⅰ and the expression of Beclin-1 protein were higher in model rats than in control rats （ P 〈 0.05 ）. Conclusion ： Atuophagy was enhanced in hippocampal neurons in rats with PTSD, which might be related to the abnormal change of hippocampal volume in PTSD.

关 键 词：创伤后应激障碍 海马 自噬 大鼠 

分 类 号：R749.5[医药卫生—神经病学与精神病学]