神经干细胞移植对缺氧缺血性脑损伤新生大鼠脑组织Foxgl基因表达的影响  

The effects of neural stem cell transplantation on Foxgl gene expression in the subgranular zone of neonatal brain tissue damaged by hypoxia-ischemia

作　　者：尚凤伟[1] 王军[1] 侯艳艳[1] 朱登纳[1] 范亚珍[1] 王俊辉[1] 张贞焕[1] 

机构地区：[1]郑州大学第三附属医院脑瘫康复科,郑州450052

出　　处：《中华物理医学与康复杂志》2013年第5期337-341,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：河南省医学科技攻关计划项目（200703066）

摘　　要：目的观察脐血源性神经干细胞（NSCs）移植对缺氧缺血性脑损伤（HIBD）新生大鼠脑组织海马齿状回颗粒细胞下层（SGZ）Foxgl基因表达的影响。方法采用密度梯度离心法分离脐血单个核细胞，进行定向诱导培养，并通过免疫细胞化学法鉴定培养细胞表达神经干细胞表面标志。将7日龄SD大鼠150只随机分为假手术组、HIBD组和NSCs组，HIBD组和NSCs组分离并永久性结扎左侧颈总动脉，假手术组只分离不结扎左侧颈总动脉，也不进行缺氧，NSCs组于造模24h后经大鼠尾静脉行脐血源性神经干细胞移植，3组大鼠分别于造模后第3、7、14、21、28天共5个时间点取材，每个时间点各取10只大鼠，应用原位杂交法检测各组大鼠SGZFoxgl基因HIBD后各时间点的表达水平。Objective To observe the effect on Foxgl gene expression in the subgranular zone （SGZ） of cerebral tissue from neonatal rats with hypoxic-ischemic brain damage （HIBD） after transplantation of neural stem cells （NSCs） derived from umbilical cord blood. Methods Mononuclear cells separated from umbilical cord blood by density gradient centrifugation were cultured with orientated induction to differentiate the NSCs. The neuronal phe- notype was identified using immunocytochemical methods. A total of 150 Sprague-Dawley rats were randomly divided into a sham-operation group, an HIBD group and an I-IIBD-NSCs group. Rats in the HIBD group and the HIBD-NSCs group were subject to ligation of the left carotid artery and then kept in a box under 8% oxygen and 92% nitrogen for 2.5 hours to establish the HIBD animal model. The artery was separated but not ligated in the sham operation group, which was not subjected to hypoxia. Twenty-four hours after the operation, the cultivated NSCs were transplanted by caudal vein injection into the rats in the HIBD-NSCs group. Rats were then sacrificed on the 3rd, 7th, 14th, 21st and 28th days after the operation. Foxgl gene expression in the SGZ was examined using in-situ hybridization meth- ods.

关 键 词：缺氧缺血性脑损伤 大脑因子1 神经干细胞 移植 新生大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]