18F-FLT和18F-FDG评价食管癌细胞照射后超早期生物学反应  

Using 18F-FLT and 18F-FDG to evaluate the ultra-early biological response of esophageal carcinomacells after irradiation

作　　者：江茂情[1] 陈皓鋆[1] 豆晓峰[1] 陈新佳[1] 林勤[1] 范文博[1] 彭添兴[1] 颜和平[1] 孙龙[1] 吴华[1] 

机构地区：[1]厦门大学附属第一医院闽南PET中心,361003

出　　处：《中华放射医学与防护杂志》2013年第2期131-134,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：福建省自然科学基金（2008-59-11）;厦门市科技计划指导性项目（3502720077056）

摘　　要：目的 比较18F-氟代脱氧胸腺嘧啶（18F-FLT）和18F-氟代脱氧葡萄糖（18F-FDG）在反映食管癌细胞受照后超早期生物学反应的差异.方法 将人食管癌Eca-109细胞分别接受5、10、15 Gy剂量X射线照射,照射后2、4、8h检测细胞对18F-FDG和18F-FLT摄取率的变化,以及细胞相对存活率和ATP表达情况的变化.结果 5 Gy照射后2、4h,细胞对18F-FDG摄取率与对照组相比分别减少了9.45％和16.4％,差异无统计学意义；但15 Gy照后2h,对18F-FDG摄取率却增加了26.5％（=3.04,P＜0.05）,其余照射组对18F-FDG摄取率均有明显减少（F=25.75,P＜0.05）.5 Gy照后2h,18F-FLT摄取率（3.65±0.41）％与对照组（4.00±0.42）％相比,差异无统计学意义,其余照射组对18F-FLT摄取率与对照组比较均有明显减少（F=33.93,P＜0.05）.在5、10、15 Gy照后2、4、8h,各组细胞相对存活率差异无统计学意义.经不同剂量照射后,细胞对18F-FLT摄取率与ATP浓度之间的相关性（r=0.887,P＜0.05）优于18F-FDG与ATP浓度之间的相关性（r=0.622,P＞0.05）.结论 18F-FDG和18 F-FLT两者均可反映食管癌细胞照射后超早期生物学反应,18F-FLT比18F-FDG能较好地反映食管癌细胞照射后超早期生物学反应.Objective To compare the difference of 3＇-deoxy-3＇-18F-fluorothymidine （ 18 F-FLT） and 2＇-deoxy-2＇-18F-fluoro-D-glucose （is F-FDG） in the ultra-early biological response of esophageal carcinoma cells after irradiation. Methods After 2, 4 and 8 h of irradiation with different doses （5, 10, 15 Gy） , the uptake ratio of 18F-FDG and 18F-FLT, survival, and ATP level of esophageal carcinoma cells were detected. Results After 2 and 4 h of 5 Gy irradiation, the cellular 18F-FDG uptake ratio was decreased by 9.45% and 16. 4%. After 2 h of 15 Gy irradiation, this uptake ratio was increased by 26.5% （t = 3.04, P 〈 O. 05）. But the 18F-FDG uptake ratios of the other experimental groups were decreased remarkably （F =25.75, P 〈0. 05）. On the other side, after 2 h of 5 Gy irradiation, the lSF- FLT uptake ratio had no statistically difference [ （3.65 ＋0. 17）% vs （4.00 -＋0. 17）%, P 〉0. 05], and lSF-FLT uptake ratios of the other groups were decreased notably （F = 33.93, P 〈 O. 05）. The relative survival of esophageal carcinoma cells after 2, 4 and 8 h of irradiation with different doses （5, 10, 15 Gy） has no significant difference. Line regression analysis indicated a significant correlation between 18F-FLT and ATP levels （r =0. 887, P 〈O. 05）, whereas a less correlation was found between lSF-FDG and ATP levels （r = 0. 622, P 〉 0.05 ）. Conclusions Both lS F-FDG and 18F-FLT can evaluate the ultra-early irradiation response of esophageal carcinoma cells, but 18F-FLT may be more specific than 18F-FDG in monitoring the ultra-early irradiation response.

关 键 词：18F-氟代脱氧葡萄糖 18F-氟代脱氧胸腺嘧啶 食管癌细胞 生物学反应 

分 类 号：R735.1[医药卫生—肿瘤]