基质金属蛋白酶抑制剂-2基因转染豚鼠形觉剥夺性近视模型生物学和巩膜形态学改变  

The effect of gene-transferred TIMP-2 on the biology and scleral morphology in form-deprivation myopia in guinea pigs

作　　者：王玲[1] 刘桂香[1] 郭强强[1] 牟丽丽[1] 高岩[1] 

机构地区：[1]青岛大学医学院附属医院眼科,266003

出　　处：《中华眼视光学与视觉科学杂志》2013年第5期290-294,共5页Chinese Journal Of Optometry Ophthalmology And Visual Science

摘　　要：目的观察基质金属蛋白酶抑制剂-2（TIMP-2）基因转染豚鼠形觉剥夺性近视模型屈光度、眼轴变化及后极部巩膜形态学改变，探讨外源性11MP-2基因对形觉剥夺性近视的治疗作用。方法实验研究。4周龄健康豚鼠60只，随机分成TIMP-2组、空质粒组、生理盐水组和对照组，每组15只。右眼为剥夺眼．采用半透明眼罩遮盖诱导轴性近视，左眼不予处理。选取TIMP-2组豚鼠左眼作为自身对照组。TIMP-2组、空质粒组、生理盐水组形觉剥夺后15d于右眼脉络膜上腔分别注射转染TIMP-2基因的脂质体、转染空质粒的脂质体和生理盐水。在注药后2、7、14d各组进行双眼屈光度、眼轴检测。然后处死豚鼠分离出巩膜。采用光镜观察巩膜的厚度，成纤维细胞的密度及细胞外基质的变化。采用透射电镜观察胶原纤维的排列、直径大小及细胞外基质的变化。对数据进行两因素方差分析及q检验。结果注药后2、7、14d TIMP-2组右眼屈光度分别为（-0．4±0．5）D、（＋0．9±0．6）D和（＋1．2±0．6）D，与空质粒组、生理盐水组和对照组比较，在注药后14d差异有统计学意义（q=5．78、5134、6．07，P〈0．05）。而与自身对照组比较，在第2、7、14天差异均有统计学意义（q=13．09、9．72、6．93，P〈0．05）；注药后2、7、14dTIMP-2组眼轴分别为（7．78±0．04）mm、（7．69±0．03）mm、（7．65±0．03）mm，其中第7、14天TIMP-2组与其他3个实验组差异有统计学意义（q7=5．51、4．43、4．82，q14=5．36、4．78、5．16；P〈0．05）。TIMP-2组注药后2、7、14d右眼胶原纤维平均直径分别为（49．7±6．8）nm、（71．3±6．0）nm和（97．8±10．4）nm，与其他3组比较在第7、14天差异均有统计学意义（q7=30．70、12．30、12．20，q14=21．80、21．50、21．20；P〈0．05），与自身对照组比较只在第2天差异有统计学意义（q=14．40，P〈0�Objective To observe axial length, refraction and morphology in form-deprivation myopia （FDM） with gene-transferred tissue inhibitors of metalloproteinase （TIMP-2） in guinea pigs. Methods Sixty four-week-old guinea pigs in this experimental study were randomly divided into 4 groups： TIMP-2 group, empty plasmid group, saline group and control group. A FDM model was established in the right eye by using a translucent plastic occluder. The left eye of the TIMP-2 group was considered as the self-control group. Gene-transferred liposome, empty plasmid- transferred liposome and saline were injected in the first 3 groups, respectively, on the 15^th day of deprivation. Refraction, axial length and the scleral structure were observed on 2, 7 and 14 days after injection. Data were analyzed using two-way analysis of variance. Results On 2, 7 and 14 days after injection, the average amounts of myopia in the TIMP-2 group were -0.4±0.5 D, 0.9±0.6 D and 1.2±0.6 D. The differences for the day 14 were statistically significant compared to the other 3 groups （q=5.78, 5.34, 6.07, P〈0.05）. Compared with the control group, the differences of day 2, 7, 14 were statistrcally significant （q=13.09, 9.72, 6.93, P〈0.05）. On 2, 7 and 14 days afterinjection, the axial lengths of the experimental group were 7.78±0.04 ram, 7.69±0.03 mm and 7.65±0.03 ram. The differences on days 7 and 14 were statistically significant compared to the control groups （q7=5.51, 4.43, 4.82, q14=5.36, 4.78, 5.16; P〈0.05）. The collagen fibrillar diameters of the selera in the posterior segment of FDM eyes were found to be distinctly bigger and the spaee between the fibers was smaller than that of the control eyes. The average diameters of the fiber in the TIMP-2 group were 49.7±6.8 nm, 71.3＋6.0 nm and 97.8±10.4 nm, respectively, 2, 7 and 14 days after injection. The differences on day 7 and 14 were statistically significant compared to the the other 3 groups （q7=30.70, 12.30, 12.20, q14=21.80, 21.50, 21.20; P〈0.05）. Compare

关 键 词：形觉剥夺性近视 基质金属蛋白酶抑制剂-2 基因转染 巩膜 豚鼠 

分 类 号：R77[医药卫生—眼科]