CCR7在结节性甲状腺肿与甲状腺癌中的表达及其与EMT关系的研究  

The relationship of CCR7 and EMT in thyroid goiter and thyroid papillary carcinoma

作　　者：吕顺增[1] 赵婧[1] 邹知耕[2] 田铧[1] 李菲[1] 房云海[3] 索宁[1] 杨春林[1] 刘书涛[1] 

机构地区：[1]山东大学医学院解剖与组织胚胎学研究所,济南250012 [2]中国医学科学院北京协和医学院 [3]山东省血液中心

出　　处：《中华普通外科杂志》2013年第5期374-377,共4页Chinese Journal of General Surgery

基　　金：基金项目：山东省自然科学基金（项目编号：ZR2010HM047）

摘　　要：目的研究并探讨趋化因子受体-7（chemokine receptor7，CCR7）在结节性甲状腺肿与甲状腺乳头状癌中与上皮间质转化（epithelia lmesenchymal transition，EMT）的关系。方法采用免疫组化检测CCR7、N-cadherin与基质金属蛋白酶-9（matrix metalloproteinase9，MMP9）在50例结节性甲状腺肿与50例甲状腺乳头状癌中的表达，分析其与患者病理资料的关系。结果CCR7、N-eadherin在结节性甲状腺肿的结节期滤泡上皮均呈高表达（分别为83．7％、90．7％），且为正相关（P〈0．01），而在胶质贮存期滤泡上皮（分别为2．0％，8．2％）与结节期纤维组织（分别为6．7％，31．1％）低表达。CCR7、N-cadherin与MMP9在甲状腺乳头状癌的淋巴结转移组的阳性表达率为87．1％，90．3％，96．8％，而无淋巴结转移组的阳性表达率为42．1％，57．9％，63．2％，差异有统计学意义（P〈0．01）；CCR7与N-cadherin、MMP9在甲状腺乳头状癌的表达呈正相关（P〈0．01）。结论CCR7在结节性甲状腺肿与甲状腺乳头状癌中均与EMT的指标具有相关性，CCR7可能参与调节滤泡上皮EMT发生。Objective To explore the relationship of chemokine receptor 7 （CCR7） and epithelial mesenchymal transition （EMT） in thyroid goiter and thyroid papillary carcinoma. Methods CCR7, N-eadherin and MMP9 were detected by immunohistochemical technique in 50 cases of thyroid goiter tissues and 50 cases of thyroid papillary carcinoma tissues. The correlation between clinical pathological data and CCR7 expression were retrospectively analyzed. Results CCR7 and N-cadherin mainly located in nodular follicular epithelium （83.7%, 90. 7% ） in thyroid goiter and correlated with each other statistically （P 〈 0. 01 ）, the expression of CCR7 and N-cadherin were low in the follicular epithelium of storage period （2. 0% , 8.2% ） and the nodular fibrous tissues （6. 7% , 31.1% ） of thyroid goiter. The expression rate of CCR7, N-cadherin and MMP9 in thyroid papillary carcinoma tissues with lymph node metastasis was 87. 1% , 90. 3%, 96. 8% respectively, while the expression rate of CCR7, N-cadherin and MMP9 in thyroid papillary carcinoma tissues without lymph node metastasis was 42. 1% , 57.9%, 63.2% respectively. CCR7, MMP9 and N-cadherin were significantly correlated with lymph node metastasis （P 〈 O. 01 ）. CCR7 was positively correlated with N-cadherin, MMP9 （ P 〈 0.01 ）. Conclusions CCR7 is associated with EMT indicators （MMP9 and N-cadherin） in thyroid goiter and thyroid papillary carcinoma. CCR7 might play a role in the genesis of EMT in the follicle epithelium.

关 键 词：甲状腺肿瘤 受体 CCR7 钙黏着糖蛋白类 基质金属蛋白酶9 上皮间质转化 

分 类 号：R736.1[医药卫生—肿瘤]