外源NO对低温胁迫下小麦幼苗果聚糖合成及FBEs表达的影响  

Effects of Exogenous Nitric Oxide on Fructan Anabolism and FBEs Expression in Wheat under Chilling Stress

作　　者：张黛静[1] 姜丽娜[1] 邵云[1] 李春喜[1] 李婷婷[1] 杨淑芳[1] 郭田[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《华北农学报》2013年第2期6-11,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家"十二五"科技支撑粮丰工程项目(2012BAD04B07);河南省科技攻关计划项目(122102310311);河南省教育厅科技研究重点项目(12B180012)

摘　　要：采用水培的方法,研究4℃低温胁迫0,24,48,96 h后,外源NO供体硝普钠(SNP)对小麦幼苗生长、可溶性总糖含量、可溶性糖各组分含量及果聚糖合成酶(FBEs)等的影响,并采用qRT-PCR方法测定小麦幼叶中FBEs基因mRNA水平的变化。结果表明,外源NO促进小麦幼苗生长,提高低温胁迫下小麦幼叶果糖、葡萄糖、低聚果糖(DP3)、可溶性总糖等含量和果聚糖:果聚糖-1-果糖基转移酶(FFT)活性,并且在mRNA水平上调节FBEs基因表达。外源NO能够通过调控FBEs基因表达,进而影响果聚糖合成酶活性,促进果聚糖积累,提高小麦抵抗低温的能力。The effects of exogenous NO on wheat（Triticum aestivum L.） growth,solubility sugar,fructan biosynthesis metabolism etc.under 4 ℃ chilling stress were investigated in seedlings,subjected to different concentrations of sodium nitroprussiate（SNP,as nitric oxide donor） for 0,24,48,96 h.The results showed that,under chilling stress,exogenous NO could induce wheat weight,fructose,glucose,low DP fructan,total soluble sugar content and fructan：fructan 1-fructosyltransferase（FFT） activity.Moreover,NO could regulate the gene expression at this level in following ways：sucrose：sucrose 1-fructosyltransferase（1-SST） and sucrose：fructan 6-fructosyltransferase（6-SFT） gene expression were inhibited by high concentration of SNP while 1-FFT was induced.Our report owing that exogenous NO alleviated the negative effects of non-freezing low temperatures by regulating FEBs and accumulating fructans.

关 键 词：NO 低温 小麦 果聚糖 FBEs表达 

分 类 号：Q78[生物学—分子生物学]