Curvularia lunata侵染不同抗性玉米品系的应答差异蛋白解析  

Proteome Changes in Corn Varieties QI319 and 7922 after Inoculating Curvularia lunata

作　　者：夏淑春[1] 张茹琴[1] 王琰 鲁闽 鄢洪海[1] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东青岛266109 [2]山东省乳山市农业局,山东威海264500 [3]烟台出入境检疫检验局,山东烟台264000

出　　处：《华北农学报》2013年第2期42-45,共4页Acta Agriculturae Boreali-Sinica

基　　金：山东省科技发展项目(2009GG10009022);山东省自然科学基金项目(ZR2011CL005);山东省"泰山学者"建设工程专项经费资助(BS2009NY040)

摘　　要：用山东莱西市玉米弯孢叶斑病菌强致病类型菌株QN-10,接种玉米抗病自交系齐319和感病自交系7922,采用蛋白质组技术分析其差异蛋白及功能。结果表明,齐319感染弯孢菌3 d后,有12个差异蛋白质斑点被诱导合成,8个为增量表达蛋白质斑点,2个为减量表达蛋白质斑点,2个为新产生蛋白质斑点;自交系7922有3个蛋白质斑点表达量增加,2个斑点表达量下降,但没有发现新的蛋白质合成。齐319差异蛋白质斑点鉴定表明,分别属于β-1,3-葡聚糖酶、F-box亮氨酸高度重复蛋白、锌指蛋白。The protein of resistant corn variety QI 319 and susceptible variety 7922 was extracted from leaves and separated using two-dimensional polyacrylamid gel electrophoresis（2-D）at 3d after inoculating prevalent race QN-10 of Curvularia lunata.In QI 319,compared with control with no pathogen inoculation,12 novel proteins were induced;In 7922,three protein spots of and two protein spots of showed increase and decrease in protein abundance,respectively,but there were no hovel protein spots induced.Among the 12 hovel protein spots induced in QI 319,six spots were identified using MALDI-TOF-MS and NCBI database searching,which were F-box and leucine-rich repeat protein,heavy metal transport/detoxification protein,beta-1,3-glucanase and zinc finger protein.

关 键 词：弯孢叶斑病菌 玉米 蛋白质组 Β-1 3-葡聚糖酶 

分 类 号：S432.1[农业科学—植物病理学]