BSA-ISCOMs对大黄鱼免疫机能及抗病力的影响  

Effects of BSA-ISCOMs on Immunity Response and Disease Resistance of Pseudosciaena crocea

作　　者：陈秀霞[1] 黄殿盛[1] 柯翎[1] 许斌福[1] 方冬兰[1] 龚晖[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《福建农业学报》2013年第3期201-205,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2011R1021-7;2009R10035-5);福建省科技平台建设项目(2009N2003);福建省海洋与渔业厅重点项目([2010]2-13)

摘　　要：利用免疫刺激复合物(ISCOMs)非特异免疫增强作用和可经黏膜呈递的特点,将BSA制备成ISCOMs(BSA-ISCOMs),大黄鱼连续口服免疫10d。免疫后第15d和30d采血,测血清部分非特异性免疫指标。结果显示,免疫后第15d,试验组血清SOD、补体C3、总蛋白含量显著高于对照组(P<0.05),γ-干扰素高于对照组,但差异不显著(P>0.05),溶菌酶则显著低于对照组(P<0.05);免疫后第30d,除溶菌酶外,试验组各指标与对照组差异不显著(P>0.05)。免疫完成后第30d,创伤弧菌(Vibrio vulnificus)攻毒结果显示,对照组死亡率100%,试验组死亡率26.7%,相对免疫保护率为73.3%。因此,口服BSA-ISCOMs在一定药效期限内能有效提高大黄鱼的免疫机能,增强抗病能力。Immune stimulating complex（ISCOMs） is an efficient mucosal delivery system which can induce non-specific immune response.Bovine serum albumin（BSA） was incorporated into ISCOMs（BSA-ISCOMs）.Pseudosciaena crocea took diets containing BSA-ISCOMs for 10 d,while the control group took diets containing PBS.At the 15th and 30th day after oral completion,serum was collected for non-specific immune parameters analysis.The results showed that at 15th day after oral completion,SOD,C3 and total protein of the fish feeding BSA-ISCOMs were significantly higher than those of the control fish（P0.05）,and interferon γ of the former was higher than that of the latter,but not significantly（P0.05）,while lysozyme of the former was significantly lower than that of the latter（P0.05）.At 30th day after oral completion,these parameters except lysozyme of the experimental group were not significantly different from the control group（P0.05）.The results of the challenge with Vibrio vulnificus at 30th day after oral completion showed that the cumulative mortality of the control and the experimental group was 100% and 26.7%,respectively,and the relative percent survival（RPS） was 73.3%.So this study indicated that BSA-ISCOMs can induce the immune response and improve the disease resistance of Pseudosciaena crocea.

关 键 词：牛血清白蛋白(BSA) 免疫刺激复合物(ISCOMs) 大黄鱼 非特异性免疫 

分 类 号：S858.3[农业科学—临床兽医学]