不同产地余甘子中没食子酸与槲皮素含量的测定  

Simultaneous Determination of Gallic Acid and Quercetin in Phyllanthus emblica of Different Areas

作　　者：郭建民[1] 王建科[1] 李玮[1] 田源红[1] 张英[1] 周林欢[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《贵州农业科学》2013年第5期61-63,共3页Guizhou Agricultural Sciences

基　　金：贵州省科学技术厅"贵州省中药;民族药炮制技术研究平台建设"[黔科合社字(2009)5001]

摘　　要：为了更好地控制余甘子药材的质量,建立高效液相色谱法同时测定余甘子中没食子酸和槲皮素含量的方法,对不同产地余甘子中没食子酸、槲皮素的含量进行测定。结果表明:余甘子中没食子酸和槲皮素含量分别在1.32～23.1μg、0.05～1.00μg呈良好的线性关系(r=0.999 7,r=0.999 7)。没食子酸平均加样回收率为101.96%,RSD为1.99%;槲皮素平均加样回收率为99.51%,RSD为2.07%。该方法简便、准确、重现性好。不同产地余甘子中没食子酸、槲皮素的含量有一定的差异。In order to better control the medicinal quality of P. emblica, contents of gallic acid and quercetin using a method for simultaneous determination gallic acid and quercetin in P. emblica by HPLC, determination of gallic acid and quercetin in P. embllca for different producing areas. The results show that： HPLC, determination gallic acid and quercetin respectively showed good linearity（r = 0. 999 3,r = 0. 999 7） in the range of 1.32~23.1 μg,0.05-1.00μg ,gallic acid average recovery was 101.96% ,RSD= 1.99%, quercetin average recovery was 99.51%, RSD=2.07%. The methed is simple,accurate and good in reproducibility . Determination of gallic acid and quercetin in P. emblica difference for different producing areas. Key words： Phyllanthus emblica ; gallic acid; quercetin; HPLC

关 键 词：余甘子 没食子酸 槲皮素 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]