肉桂酸对人肺腺癌细胞诱导分化的实验研究  

Induced differentiation of human lung adenocarcinoma cells by cinnamic acid

作　　者：王涛[1] 金戈[2] 王淑梅[1] 董子明[2] 

机构地区：[1]河南医科大学第一附属医院呼吸内科,河南郑州450052 [2]河南医科大学基础医学院,河南郑州450052

出　　处：《癌症》2000年第8期782-785,共4页Chinese Journal of Cancer

摘　　要：研究肉桂酸对人肺腺癌细胞的诱导分化作用。方法 :在细胞培养中 ,用不同浓度的肉桂酸处理人肺腺癌A549细胞 ,进行细胞群体增殖动力学检测、流式细胞计量术 (FCM )测定来观察诱导分化作用效应。结果 :肉桂酸能明显抑制A549细胞增殖和促进细胞分化 (P<0 05) ;细胞在软琼脂中的集落形成率明显下降 (P<0 05) ;FCM显示细胞周期移行被阻滞于G1/G0 期。结论 :肉桂酸具有使人肺腺癌细胞逆转的作用 。Objective: To investigate the effect of cinnamic acid (CINN) on differentiation induction of a human lung adenocarcinoma cell subline (A549) Methods: A549 cells were treated with CINN in vitro The proliferation rate, mitotic index, colony forming ability in semisolid agar culture were tested The cell cycle was detected by flow cytometry(FCM) Results: CINN treatment could markedly inhibit proliferation and mitosis(P<0 05), reduce colony forming rate (P<0 05) CINN may inhibit DNA synthesis and mitosis of A549 cells, and arrest the cells in the G1/G0 phase Conclusion: This study indicated that CINN could induce the reversion of malignancy of A549 cells and provided experimental data for potential use of CINN in cancer treatment

关 键 词：肉桂酸 肺腺癌 A549细胞 诱导分化 

分 类 号：R734.2[医药卫生—肿瘤]