七叶皂苷钠对脑缺血再灌注损伤大鼠MG、iNOS表达的影响  

Effects of aescine on the expression of microglia and iNOS following cerebral ischemia-reperfusion injury in rats

作　　者：杜家琇[1] 王明强[1] 娄季宇[2] 

机构地区：[1]郑州人民医院,郑州450000 [2]郑州大学二附院,郑州450014

出　　处：《中国实用神经疾病杂志》2013年第9期33-34,共2页Chinese Journal of Practical Nervous Diseases

摘　　要：目的观察七叶皂苷钠对大鼠脑缺血再灌注损伤后小胶质细胞(MG)及诱导型一氧化氮合酶(iNOS)表达的影响,探讨其脑保护作用的可能机制。方法将SD大鼠随机分为假手术组、对照组、七叶皂苷钠组。改良线栓法制备建立局灶性脑缺血-再灌注动物模型(MCAO模型),分别于手术后1d、3d、7d免疫组化法观察大鼠海马一区OX-42及iNOS的表达情况。结果对照组、七叶皂苷钠组3d时OX-42达峰,7d时明显降低;iNOS 1d达峰,3d明显下降,7d时接近正常。七叶皂苷钠组优于对照组(P<0.05)。结论七叶皂苷钠可能通过抑制小胶质细胞的活化和iNOS的表达,对大鼠脑缺血再灌注损伤发挥脑保护作用。Objective To observe the effects of aescine on the expression of microglia and iNOS following cerebral ischemi- a-reperfusion injury in rats, and to investigate the protective mechanism of brain. Methods Rats were randomly divided into the sham-operative group, normal group and aescine group. The focal cerebral ischemia-reperfusion injury models were made by suture-occluded method, namely the middle cerebral artery occlusion （MCAO） model. The expressions of OX-42 and iNOS in the rats hippocampal CA1 was detected by immunohistochemistry staining at 1 day, 3 days and 7 days after operation. Results In the normal group and aescine group, the number of OX-42 positive cells peaked at 3rd day of reperfusion, and it decreased at 7th day. iNOS peaked at 1st day, and then decreased gradually at 3rd day. It wlosed to normal at 7th day. Compared with nor- mal group, the expressions of OX-42 and iNOS in Cyclosporine group were reduced （P（0.05）. Conclusion The aescine could significantly reduce the expressions of OX-42 and iNOS. It would have protective effect on ischemia-reperfusion injury in rats.

关 键 词：七叶皂苷钠 缺血再灌注损伤 小胶质细胞 诱导型一氧化氮合酶 

分 类 号：R-332[医药卫生]