两株紫色红曲菌根癌农杆菌介导的T-DNA转化子的生物学特性  

The biological characters research of two transformants from the transformants library of Monascus purpureus by Agrobacterium-mediated DNA transfer

作　　者：曹丽凌[1] 齐育平[1] 梁帅帅[1] 施晓莉[1] 蒋冬花[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2013年第2期217-223,共7页Journal of Zhejiang Normal University:Natural Sciences

基　　金：国家自然科学基金资助项目(31070008);浙江省自然科学基金资助项目(Y3090343)

摘　　要：从根癌农杆菌(Agrobacterium tumefaciens)介导的紫色红曲菌(Monascus purpureus)菌株S的转化子库中筛选出2株产红曲色素性能变化显著且遗传稳定的转化子S62和S158,分别对其菌落形态、显微结构、菌落生长速度、遗传稳定性、潮霉素抗性及其产色素和桔霉素的能力等进行了分析.结果表明,2株转化子的生物学特性均发生了显著变化,且发酵后红曲色素的色价和桔霉素的产量降低.其中:转化子S62液态发酵后色价为2.71 U/mL,仅为原始菌株S色价的0.04倍;转化子S158发酵后色价为17.62 U/mL.2 transformants of genetic stability from Monascus purpureus transformants library mediated by Agrobacterium tumefaciens,which were screened with significant changes in producing Monascus pigment were studied.Characters of the transformants,including colonial and microscopical morphology,growth rate,genetic stability,hygromycin resistance,pigments and citrinin production capacity were analyzed.The results indicated that the biological characters of transformants were significantly different from the wild strain S.Under liquid ferment,the color value of transformant S62 was 2.71 U/mL,which was 0.04 fold of the origin,and the transformant S158′s was 17.62 U/mL.

关 键 词：紫色红曲菌 根癌农杆菌 转化子 红曲色素 

分 类 号：Q93[生物学—微生物学]