酪氨酸转氨酶法制备L-高苯丙氨酸  

Synthesis of L-homophenylalanine by tyrosine aminotransferase method

作　　者：李志强[1] 王潇莹[2] 娄晓月[2] 刘冰滢[2] 张朗[2] 游松[2] 

机构地区：[1]沈阳双鼎制药有限公司,辽宁沈阳110179 [2]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2013年第5期399-402,408,共5页Journal of Shenyang Pharmaceutical University

基　　金：本溪医药产业园手性药物生物催化平台建设(2010ZX09401-304-103)

摘　　要：目的为抗高血压新药的共同中间体L-高苯丙氨酸的制备奠定基础。方法构建工程菌E.coli BL21(DE3)pET 30a-tyrB,乳糖诱导表达酪氨酸转氨酶;利用酪氨酸转氨酶对底物2-氧-4-苯基丁酸进行转化;利用HPLC、MS、1H-NMR进行产物检测与结构鉴定。结果构建了工程菌E.coliBL21(DE3)pET 30a-tyrB,乳糖诱导酪氨酸转氨酶表达,制备得到L-高苯丙氨酸。结论利用转氨酶催化法可以高效制备L-高苯丙氨酸。Objective To lay the foundation for efficient preparation of L-homophenylalanine,which is a common intermediate for antihypertensive drugs.Methods The engineering bacteria E.coli BL21（DE3）pET 30a-tyrB was established first,then the tyrosine aminotransferase was induced by lactose and the transformation of 2-oxo-4-phenyl butyric acid to L-homophenylalanine was accomplished by the enzyme mentioned above.HPLC,MS,1H-NMR were used to indentify the products.Results The engineering bacteria were established and L-homophenylalanine was synthesized by catalysis of tyrosine aminotransferase.Conclusions The aminotransferase is a potential and effective tool for the synthesis of L-homophenyalanine.

关 键 词：酪氨酸转氨酶 L-高苯丙氨酸 转化 乳糖 诱导 

分 类 号：R91[医药卫生—药学]