InDel指数法评价水稻籼粳成分的验证  

Verification of InDel Index in Evaluating Indica-japonica Content of Rice

作　　者：姚国新[1,2] 黄文超[2] 

机构地区：[1]湖北工程学院生命科学技术学院,湖北孝感432000 [2]武汉大学生命科学学院,湖北武汉430072

出　　处：《河南农业科学》2013年第4期24-27,共4页Journal of Henan Agricultural Sciences

基　　金：湖北省自然科学基金项目(2012FFB00504);湖北工程学院博士科研启动基金项目(HE2010002)

摘　　要：传统的籼粳分类采用程式指数法,需要大田种植、鉴定,所需时间长。利用分子标记可以快速、准确鉴定籼粳成分。Lu等开发了1套34对InDel标记,用于快速鉴定籼粳的成分。为了进一步验证该方法的准确性,利用734对SSR标记和34对InDel标记分别对籼粳杂交后代材料进行籼粳成分鉴定,结果2套标记鉴定结果较为吻合,证明该套InDel标记完全可以用于水稻籼粳成分的快速准确鉴定。The traditional way for indica-japonica classification is Chen＇s index method, which needs a long time for field-planting and identification. The molecular marker can be used to identify the subspecies content rapidly and accurately. Lu et al developed a set of InDel markers with 34 pairs of primers for rapid identification of indica-japonica content. In order to verify the accuracy of InDel index,Chen＇s 734 SSR markers and Lugs 34 InDel markers were used to evaluate the indica-japonica content. The results showed that the accuracy of the two methods was almost identical,indicating that the 34 InDel markers could be used to identify the indica-japonica content rapidly and accurately.

关 键 词：快速鉴定 籼粳成分 INDEL标记 SSR标记 验证 

分 类 号：S511[农业科学—作物学]