GA_3和PP_(333)对榛子成花的影响及其作用机制  

Effects of GA_3 and PP_(333) on Flowering of Hazelnut and Their Functional Mechanisms

作　　者：孟晓庆[1] 侯智霞[1] 张兰[2] 苏淑钗[1] 朴蕾[1] 王培培[1] 王冲[1] 

机构地区：[1]北京林业大学林学院,北京100083 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《河南农业科学》2013年第4期115-118,共4页Journal of Henan Agricultural Sciences

基　　金：博士后特别资助基金项目(200902052);国家林业局重点项目(2011-03)

摘　　要：利用环剥摘叶处理的方法确定榛子雄花芽生理分化时期,并分别喷施GA3(100mg/L)和PP333(800mg/L和1g/L),研究其对榛子雌花、雄花序数量以及成花基因LFY表达的影响。结果表明,榛子雄花芽生理分化期为4月22日至5月28日。GA3100mg/L处理使雄花序数量显著增加,雌雄比例降低。PP3331g/L处理则使雌花数量显著增加,雌雄比例升高。荧光定量PCR结果显示,GA3促进了榛子LFY基因的表达,PP333抑制了榛子LFY基因的表达,推测榛子成花转变与榛子LFY基因有密切关系。The stage of physiological differentiation of male flower-bud was studied on hazel by using the methods of the band girdle and leaf removal methods. GA3 （100 mg/L） and PP333 （800 mg/L and 1 g/L） was sprayed on the leaves of hazel respectively, to research on the amount of hazelnut male,female flowers and the expression of the key gene LFY in leaf using the Real-time qPCR method. The results showed that the stage of physiological differentiation of male flower- bud was from April 22th to May 28th. Spraying GA3 could increase the number of male inflorescence and decrease the ratio of female to male. PP333 could increase the number of female inflorescence and the ratio of female to male rose. Real-time qPCR results showed that the expression of the key gene LFY was promoted by GA3 and inhibited by PP33a treatment. From these,a close relation between hazelnut flowering transition and LFY gene was conjectured.

关 键 词：榛子 花芽分化 GA3 PP333 LFY基因 实时定量PCR 

分 类 号：S664.4[农业科学—果树学] S727.3[农业科学—园艺学]