不同穴组电针对局灶性脑梗死大鼠神经行为学及Neurocan表达的影响  

Effects of Electroacupuncture at Different Acupoints on Neuroethology and Expression of Neurocan after Focal Cerebral Infarction in Rats

作　　者：钟艺华[1] 唐显军[1] 李光勤[2] 黄浩然[2] 

机构地区：[1]重庆市肿瘤研究所神经内科,重庆市400031 [2]重庆医科大学附属第一医院神经内科,重庆市40001

出　　处：《中国康复理论与实践》2013年第5期444-447,共4页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的比较电针不同经络穴组对局灶性脑梗死大鼠神经行为学及neurocan表达的干预效果.方法将健康成年雄性Sprague-Dawley大鼠85只随机分为5组：假手术组（n=5）、模型组（n=20）、水沟-百会组（n=20）、肝俞-肾俞组（n=20）和足三里-曲池组（n=20）;模型组和各电针组线栓法制作大鼠大脑中动脉闭塞模型,随机再分为脑缺血1 d、3 d、7 d、14 d和21 d共5个亚组.神经行为学采用Longa评分,RT-PCR及免疫组化测定缺血侧皮质neurocan mRNA和蛋白表达.结果假手术组无神经功能障碍;各电针组大鼠在缺血3 d后神经行为学评分逐渐恢复,7~21 d时较模型组明显改善（P〈0.05）.模型组neurocan mRNA和蛋白表达在缺血1 d后逐渐增强,14 d达到峰值,21 d时有所下降,但仍维持高水平表达（P〈0.01）;各电针组在缺血3 d后neurocan mRNA和蛋白表达均较模型组低（P〈0.05）,其中水沟-百会和肝俞-肾俞组在缺血14 d时neurocan mRNA和蛋白表达较足三里-曲池组低（P〈0.05）.结论局灶性脑梗死大鼠缺血侧皮质neurocan mRNA和蛋白表达明显上调.急性期给予电针刺激能下调其表达水平,改善神经行为学评分;其中电针水沟-百会穴和肝俞-肾俞穴较足三里-曲池穴治疗效果更好.Objective To compare the effects of electroacupuncture （EA） at different acupoints on the neuroethology and expression of neurocan after focal cerebral infarction in rats. Methods The healthy male adult Sprague-Dawley rats were randomly divided into sham group （n=5）, model group （n=20）, Shuigou-Baihui （SB） group （n=20）, Ganshu-Shenshu （GS） group （n=20） and Zusanli-Quchi （ZQ） group. All the rats were modeled with middle cerebral artery occlusion （MCAO） except sham group, and were randomly divided into 5 sub-groups as 1 d, 3 d, 7 d, 14 d and 21 d after cerebral ischemia. Their neuroethology was evaluated with Longa＇s score, and the expressions of neuro-can mRNA and protein were detected with RT-PCR and immunohistochemistry in ischemic cortex. Results No dysfunction was found in the sham group. The neurological function recovered gradually 3 d after modeled, and improved significantly 7~21 d after modeled in each EA group compared with those in the model group （P〈0.05）. The expression of neurocan mRNA and protein gradually increased 1 d after mod-eled in the model group, peaked 14 d after modeled, and remained high level 21 d after modeled （P〈0.01）. The expressions of neurocan mRNA and protein were less in each EA group than in the model group 3 d after modeled （P〈0.05）, and were less in the SB group and GS group than in ZQ group （P〈0.05）. Conclusion The expression of neurocan increased significantly in ischemic cortex. EA may down-regu-late the expression of neurocan after acute cerebral infarction, and improve the neuroethology. EA at SB and GS is more effective than at ZQ.

关 键 词：脑梗死 电针 NEUROCAN 轴突再生 神经行为学 

分 类 号：R743.3[医药卫生—神经病学与精神病学]